文献类型：期刊文献

中文题名：免疫球蛋白缓释微囊的制备、结构表征与性能研究

英文题名：Study on the Preparation, Structure, and Properties of Immunoglobulin Sustained-release Microcapsules

作者：龚平[1];吴锦霞[2];谭天伟[2]

第一作者：龚平

机构：[1]北京联合大学生物化学工程学院;[2]北京化工大学生命科学与技术学院

第一机构：北京联合大学生物化学工程学院

年份：2005

卷号：25

期号：12

起止页码：15-19

中文期刊名：中国生物工程杂志

外文期刊名：Progress In Biotechnology

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：免疫球蛋白;缓释微囊;扫描电镜

外文关键词：Immunoglobulin Sustained-released microcapsules SEM

摘要：微囊化是实现生物药物制剂缓控释放的最重要方法之一。以乙基纤维素(EC)为囊材,采用物理化学方法制备了免疫球蛋白乙基纤维素微囊。用扫描电镜(SEM)考察了微囊的孔结构和形貌特征,并通过体外溶出实验考察了微囊的缓释效果。结果表明,在给定制备条件下制得的微囊圆整度好,孔径分布范围较窄,在模拟条件下具有良好的缓释性能。微囊最佳制备工艺条件如下:囊心囊材比2.5∶1,30℃下反应6h,搅拌速率250r/min,吐温80用量8ml。
Microcapsulation was one of the most important methods by which the sustained- release and controlled- release of biopharmaceuticals could be readily achieved. The Ig microcapsules were prepared by a physical chemistry method, ethycellulose used as wall materials. The pore structures and morphology of the Ig microcapsules were investigated by SEM, and their controlled-release behavior was studied with the tests in vitro. The results showed that the resulted microcapsules, which were prepared under the given conditions, were round, the distribution of the particle size was narrow, and the better sustained-release character was obtained under the simulated conditions. The optimum condition of preparing Ig microcapsules was as following ： the ratio of wall and core materials being 2.5：1 ,reacting at 30℃ for 6h, stirring at 250r/min, and 8ml of Tween-80 added.