文献类型：期刊文献

中文题名：酶解-超声法对猪苓多糖正交优选提取及抗氧化活性的初步实验研究

英文题名：Optimization of Enzymatic Coupled with Ultrasonic-assisted Extraction of Polysaccharide from Polyporus umbellatus and Its Antioxidant Activity

作者：张元[1];冯琼[1];杨晓方[1];谷春秀[1];刘红梅[1]

第一作者：张元

机构：[1]北京联合大学生物化学工程学院

第一机构：北京联合大学生物化学工程学院

年份：2015

卷号：27

期号：9

起止页码：1657-1662

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：北京市财政专项(PXM2014_014209_07_000008);北京市教育委员会科学研究支助项目(SQKM201411417001)

语种：中文

中文关键词：猪苓;猪苓多糖;纤维素酶;果胶酶;超声提取

外文关键词：Polyporus umbellatus polysaccharide; cellulose; pectlnase; ultrasonic extraction

摘要：利用复合酶解法正交优化猪苓多糖的提取工艺,并附加超声波辅助处理。酶法-超声提取的多糖进行体外抗氧化活性的初步评价。结果表明对猪苓多糖的酶法提取产生影响的因素主要有:加酶比例、温度、p H值和时间,各因素对猪苓多糖提取效果的影响由大到小依次为:温度>p H值>酶用量>提取时间。最佳提取工艺为:纤维素酶∶果胶酶复合酶(比例:1∶1,以质量计为猪苓的1%),酶解温度50℃,p H=6.5,反应时间60 min,收率为4.31%。酶法提取后加超声提取超声处理30 min,多糖得率提高至5.48%。提取的猪苓多糖进行体外抗氧化活性的初步实验,表明其抗氧化能力与Vc大致相当。因此酶法-超声波辅助提取猪苓中的猪苓多糖的具有工艺简便易行、反应条件温和、绿色环保等优点,该法提取的多糖具有抗氧化活性能力。
The extraction process of Polyporus umbeUava polysaccharide （PUPS） was optimized by orthogonal test via multiplex-enzyme hydrolysis with ultrasonic-assisted treatment. The in vitro antioxidant activity of polysaceharides extrac- ted through enzymatic-ultrasonic process were evaluated preliminarily. The results showed that the proportion of en- zymes, temperature, pH value and time affected the extraction of PUPS through enzymatic hydrolysis. The order of impact of various factors on PUPS extraction was temperature 〉 pH value 〉 enzyme dosage 〉 extraction time. The highest yield of 4. 31% was obtained in the 1：1 ratio of cellulase： pectinase （ by mass as Polyporus 1% ） at 50 ℃ and pH 6.5 for 60 rain. The yield of polysaccharide increased to 5.48% with additional 30 min of ultrasonic treatment after enzymatic ex- traction. The in vitro antioxidant activity of the extracted PUPS was proved to be similar to that of Vc. Therefore, the en- zymatic-ultrasonic extraction of PUPS had the advantage of simple process,mild reaction condition and environmental protection. The extracted polysaccharides had antioxidant activity.