文献类型：期刊文献

英文题名：Optimization of Electrochemiluminescence Experimental Conditions for Metoclopramide Determination Based on Response Surface Methodology

作者：Yang, Fuxiu[1];Zhang, Wenjuan[1];Liu, Baining[1,2];Ji, Yizhi[1,2];Zhao, Youxi[1,2];Zhou, Kaowen[1,2]

第一作者：Yang, Fuxiu

通讯作者：Zhou, KW[1];Zhou, KW[2]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2020

卷号：15

期号：8

起止页码：8145-8155

外文期刊名：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000560655800080)】；

基金：This work was supported by Beijing Natural Science Foundation of China (Grant No.2152013) and Postgraduate Funding Project of Beijing Union University (2019-068).

语种：英文

外文关键词：Electrochemiluminescence; Metoclopramide; Response surface methodology; Optimization

摘要：Based on the strong enhancement effect of metoclopramide on the electrochemiluminescence signal of Ru(bpy)(3)(2+) on the platinum electrode, a novel method for the sensitive detection of metoclopramide was established. Based on the single factor experiments, the interactions among the three factors were investigated by response surface methodology. Finally, the optimal experimental conditions for the determination of metoclopramide in a limited number of experiments are: detection potential 1.18 V, phosphate buffer solution concentration 39.82 mmol/L and pH 7.69. These conditions can improve the sensitivity of the method by 4.5%, which is very important for the determination of trace components. The detection limit (3a) of the method was 4.0 x 10(-3) mg/L, the linear range was 0.02-36.56 mg/L, the correlation coefficient was 0.9991, and the recoveries were 97.3%-102.4%. This method is simple, rapid, sensitive and with less injection.