文献类型：期刊文献

英文题名：Total Glycosides of Cistanche deserticola attenuates DSS-induced inflammatory bowel disease by regulating intestinal environmental homeostasis

作者：Zhang, Shao-shi[1,2];Feng, Duo[1,2];An, Jian-zhang[1,2];Zhao, Jian[1,2];Zhao, Jiang-yan[1,2];Guo, Yu[1,2];Jiang, Yong-jun[3];Yan, Wen-jie[1,2]

第一作者：Zhang, Shao-shi

通讯作者：Yan, WJ[1];Yan, WJ[2]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Bioact Subst & Funct Food, Beijing 100191, Peoples R China;[2]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[3]Inner Mongolia Sankou Biotechnol Co Ltd, Ordos 017000, Inner Mongolia, Peoples R China

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Bioact Subst & Funct Food, Beijing 100191, Peoples R China;[2]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;[114172]北京联合大学应用文理学院;

年份：2025

卷号：2

期号：2

外文期刊名：FOOD & MEDICINE HOMOLOGY

收录：WOS:【ESCI(收录号:WOS:001782511100001)】；

基金：This study was supported by the National Key Research and Development Program of China (No. 2023YFF1103802).

语种：英文

外文关键词：Total Glycosides; intestinal disease; gut microbiota; echinoside; verminoside

摘要：This study investigates the protective effects of total glycosides of Cistanche (TGs) on the intestinal tract of inflammatory bowel disease (IBD) mice induced by dextran sulfate sodium (DSS) from the perspectives of immune regulation and intestinal homeosta. We evaluated the IBD disease activity index (DAI) and histopathological changes of the colon tissue in mice, and validated the involvement of NF-kappa B and JAK2 in TGS-mediated IBD regulation by real-time quantitative PCR (qRT-PCR), serum enzyme-linked immunosorbent assay (ELISA), and TUNEL Cell Apoptosis Detection. Finally, we analyzed the changes in the intestinal microbiome. The results show that TGs can effectively improve the disease activity index of sick mice, alleviate the damage to the epithelial barrier, improve the morphology of the intestinal epithelial tissue, and reduce the infiltration of inflammatory cells in the damaged colon. TGs inhibit the activation of the two key signal targets of NF-kappa B and JAK2, reduce the expression of inflammatory factors such as IL-1 and TNF-alpha, and increase the expression of anti-inflammatory factor IL-10. In addition, TGs reduce the abundance of Proteobacteria, increase the proportion of Bacteroidetes and Thermotogae, and upregulate the numbers of Bacteroides, Lachnospiraceae_NK4A136_group, and Oscillospiraceae to alleviate intestinal microbiome dysbiosis and restore intestinal microenvironmental homeosta. In summary, these research results suggest that TGs can improve IBD by alleviating inflammation and restoring intestinal homeosta, providing experimental evidence for the mechanism of Cistanche intervention in IBD.