文献类型：期刊文献

英文题名：Characterization of simultaneous removal of nitrogen and phosphorus by novel Raoultella ornithinolytica strain YX-4 and application in real farm wastewater treatment

作者：Fu, Weilai[1,3,4];Zhao, Youxi[2];Wang, Qiang[1];Yu, Xuan[1];Song, Ziyang[3];Duan, Peifeng[1];Xu, Meijuan[1];Zhang, Xian[1];Rao, Zhiming[1]

第一作者：Fu, Weilai

通讯作者：Zhang, X[1]

机构：[1]Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China;[2]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[3]Jiangsu Aishengmu Feed Co Ltd, Yancheng 224300, Peoples R China;[4]Beijing BEONY Technol Co Ltd, Beijing 102200, Peoples R China

第一机构：Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China

通讯机构：[1]corresponding author), Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China.

年份：2024

卷号：391

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20234414996198);Scopus(收录号：2-s2.0-85175247453);WOS:【SCI-EXPANDED(收录号:WOS:001102811500001)】；

基金：This work was supported by the National key research and development program of China (2021YFC2100900) , National Natural Science Foundation of China (32170031) , Project 111 (111-2-06) and the Industrial Development Program of Jiangsu Province "Collaborative Innovation Center for Advanced Industrial Fermentation".

语种：英文

外文关键词：Raoultella ornithinolytica strain YX-4; Nitrogen/phosphorus balance analysis; Denitrification of phosphorus accumulating; organisms; Farm wastewater treatment

摘要：A novel strain having ability to simultaneously remove ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and phosphorus was isolated from swine farm wastewater and was identified as Raoultella ornithinolytica YX-4 (NCBI accession number: OR646540). Nitrogen and phosphorus balance analysis combined with amplification of key enzyme genes of metabolic pathways revealed that the strain possess heterotrophic nitrification, aerobic denitrification, phosphorus accumulation and assimilation pathways. Significant removal of ammonium nitrogen, nitrate nitrogen and nitrite nitrogen were achieved (99%, 97% and 93% respectively) with optimal culture conditions. The transcript level of key enzyme genes was detected at different incubation period, and significant up-regulation of glnA, narI, narH, nirB, nirD, ppk1, and ppk2 was noted. This is the first report of the denitrification of phosphorus accumulating organisms R. ornithinolytica and reveals tangible results of key enzyme gene expression during real wastewater treatment.