文献类型：期刊文献

英文题名：Rapid Characterization of Chemical Components in Edible Mushroom Sparassis crispa by UPLC-Orbitrap MS Analysis and Potential Inhibitory Effects on Allergic Rhinitis

作者：Wang, Zhixin[1];Liu, Jingyu[2];Zhong, Xiangjian[1];Li, Jinjie[1];Wang, Xin[1];Ji, Linlin[1];Shang, Xiaoya[1]

第一作者：Wang, Zhixin

通讯作者：Wang, ZX[1];Shang, XY[1]

机构：[1]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Food, 191 Beitucheng West Rd, Beijing 100191, Peoples R China;[2]Shanxi Agr Univ, Coll Food Sci & Engn, 1 Mingxian South Rd, Jinzhong 030801, Taigu County, Peoples R China

第一机构：北京联合大学生物化学工程学院|北京联合大学应用文理学院

通讯机构：[1]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Food, 191 Beitucheng West Rd, Beijing 100191, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;[114172]北京联合大学应用文理学院;

年份：2019

卷号：24

期号：16

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000482998900037)】；

基金：This work was supported by the Key Projects of Scientific Research Program of Beijing Municipal Commission of Education (Grant No. KZ201811417049).

语种：英文

外文关键词：Sparassis crispa; identification; UPLC-MS; allergic rhinitis (AR); anti-inflammatory activity

摘要：Sparassis crispa is a kind of edible fungus widely grows in the north temperate zone, which shows various medicinal properties. Due to the complexity of chemical constitutes of this species, few investigations have acquired a comprehensive configuration for the chemical profile of it. In this study, a strategy based on ultra-high performance liquid chromatography (UPLC) combined with Orbitrap mass spectrometer (MS) was established for rapidly characterizing various chemical components in S. crispa. Through the summarized MS/MS fragmentation patterns of reference compounds and systematic identification strategy, a total of 110 components attributed to six categories were identified for the first time. Moreover, allergic rhinitis (AR) is a worldwide inflammatory disease seriously affecting human health, and the development of drugs to treat AR has been a topic of interest. It has been reported that the extracts of S. crispa showed obvious inhibitory effects on degranulation of mast cell- and allergen-induced IgE and proinflammatory mediators, but the active components and specific mechanism were still not clear. Src family kinases (SFKs) participate in the initial stage of allergy occurrence, which are considered the targets of AR treatment. Herein, on the basis of that self-built chemical database, virtual screening was applied to predict the potential SFKs inhibitors in S. crispa, using known crystal structures of Hck, Lyn, Fyn, and Syk as receptors, followed by the anti-inflammatory activity evaluation for screened hits by intracellular calcium mobilization assay. As results, sparoside A was directly confirmed to have strong anti-inflammatory activity with an IC50 value of 5.06 +/- 0.60 mu M. This study provides a useful elucidation for the chemical composition of S. crispa, and demonstrated its potential inhibitory effects on AR, which could promote the research and development of effective agents from natural resources.