文献类型：期刊文献

中文题名：Determination of 19 polyphenolic compounds in tea by ultra-high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry

作者：Jian Li[1,2];Junmei Ma[3,4];Yan Zhang[3,4];Lei Zheng[1]

第一作者：Jian Li;李健

机构：[1]School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China;[2]College of Applied Arts and Science,Beijing Union University,Beijing 100191,China;[3]Hebei Key Laboratory of Forensic Medicine,College of Forensic Medicine,Hebei Medical University,Shijiazhuang 050017,China;[4]Hebei Food Safety Key Laboratory,Hebei Food Inspection and Research Institute,Shijiazhuang 050227,China

第一机构：School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China

年份：2022

卷号：11

期号：3

起止页码：719-726

中文期刊名：食品科学与人类健康（英文）

外文期刊名：Food Science and Human Wellness

收录：Scopus

基金：supported by National Key Research and Development Program of China(2018YFC1603400)。

语种：英文

中文关键词：Ultra-high performance liquid chromatography;Quadrupole-time of flight mass spectrometry;Tea;Polyphenols

摘要：A rapid method was presented for the determination of 19 polyphenols in tea by ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry(UPLC-Q-TOF MS).Tea samples were extracted by 50%(V/V)ethanol,then separated by Waters Acquity BEH C18 column using a binary solvent system composed of acetonitrile and water(0.1%formic acid)by gradient elution.The analytes were determined by Q-TOF MS in TOF MS and information dependent acquisition(IDA)-MS/MS mode.The results showed that mass accuracy error of the 19 polyphenols were lower than 5.0×10^(-6),good linear relationship was got in range of 0.2–500μg/L and correlation coefficient was higher than 0.9990.The LOD was in the range of 0.002–0.100 mg/kg and the LOQ was in the range of 0.004–0.200 mg/kg.Recovery of the method was in range of 78.4%–109.2%with spike levels of 0.004–2.000 mg/kg,relative standard deviations were lower than 10%.The method was simple,rapid and accurate.It could be used for the rapid screening and quantitative analysis of 19 polyphenols in tea.