文献类型：期刊文献

英文题名：Isolation and characterization of a novel bacterium Pseudomonas aeruginosa for biofertilizer production from kitchen waste oil

作者：Li, Ying[1];Cui, Ting[1];Wang, Yaxin[1];Ge, Xizhen[1]

第一作者：李映

通讯作者：Ge, XZ[1]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2018

卷号：8

期号：73

起止页码：41966-41975

外文期刊名：RSC ADVANCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000454837400033)】；

基金：This study was financially supported by Joint Funding of Beijing Municipal Natural Science Foundation-Beijing Municipal Education Commission, National Key R&D Program of China [2017YFD0201105] and Premium Funding Project for Academic Human Resources Development in Beijing Union University [BPHR2017DZ07].

语种：英文

外文关键词：Agricultural robots - Bacteria - Biodegradation - Cultivation - Degradation - Fatty acids - Fertilizers - Gas chromatography - Kitchens - Mass spectrometry - Petroleum industry - Probiotics

摘要：Kitchen waste oil is composed of long chain triglycerides (LCTs) that has high energy density. However, it is hard to be degraded by microbes, thereby leading to increasing levels of environmental pollution due to landfill disposition. In this study, we isolated and characterized a novel bacterium Pseudomonas aeruginosa PA-3 that could convert kitchen waste oil into biofertilizer. PA-3 could survive on trilaurin or kitchen waste oil as the sole carbon source, and 10g L-1 trilaurin or kitchen waste oil was completely consumed within 7 days. Interestingly, the degradation products of kitchen waste oil can be used as biofertilizer in promoting cabbage growth. The plant height, leaf area and stem diameter of cabbage plants were all increased with the addition of kitchen waste oil cultivation products into the soil. Kitchen waste oil degradation products were analyzed by gas chromatography mass spectrometry (GC-MS), and short chain alcohols or fatty acids were observed to be the main products. To unravel the mechanism underlying the accelerated cabbage growth, bacterial diversity of the soil was investigated after using this biofertilizer. Results showed that agricultural probiotics accumulated with the addition of kitchen waste oil cultivation products. Finally, the whole genome of PA-3 was sequenced and analyzed, which showed the existence of a complete -oxidation pathway in the genome of PA-3. To our knowledge, this is the first study on kitchen waste oil degradation and re-utilization by bacteria, which provides a new method for waste source re-utilization.