文献类型：期刊文献

英文题名：RETRACTED ARTICLE: The anti-damage ability of the Bamboo leaves extract

作者：Liu, Xuan[1,2]; Zhao, Xiaohong[1]; Mi, Shenquan[1]; Luan, Na[1]; Mei, Jing[1]

第一作者：Liu, Xuan

通讯作者：Zhao, X.

机构：[1] College of Applied Arts and Sciences, Beijing Union University, Beijing100083, China; [2] Insititute of Textile and Clothing, Hong Kong Polytechnic University, Hong Kong, Hong Kong

第一机构：北京联合大学应用文理学院

年份：2011

外文期刊名：5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011

收录：EI(收录号：20112814141709)

语种：英文

外文关键词：Antioxidants - Ascorbic acid - Bamboo - Biomedical engineering - Cell proliferation - Cells - Chemical activation - Chemiluminescence - Copper compounds - Cytology - Damage detection - Free radicals - Pathology

摘要：Objective In this paper, extracts of bamboo leaves, Phyllostachys praecox (BLE)have been selected to explore their effect on cell proliferation, antioxidant capacity, anti-DNA damage and anti-inflammation injury ability in vitro. Methods DPPH free radical scavenging test and ultra-weak chemiluminescence assay were applied to measure the antioxidant capacity of the bamboo leaves extract; MTT assay was used to detect the cell proliferation effect of the extracts and PM2.5; Comet assay was applied to the DNA strand breakage detection of the H2O2-induced RAW264.7 and anti-DNA damage of BLE. Western blot method was used to detect the expression of NF-kB in A549 cell. Results (1)BLE had the abilities of removing DPPH free radicals and IC50 was 7.02mg/L, which was lower than that of ascorbic acid (2.43mg/L). (2)BLE decrease and delay the DNA damage significantly in CuSO4-Phen-Vc-H2O2-DNA reaction of ultra-weak chemiluminescence system. (3)The tail DNA content was decrease by BLE significantly on H2O2-induced RAW264.7. (4)BLE showed little cytotoxicity for A549 cell and can resist the changes in cell viability that caused by different concentrations of PM2.5.(5)100mg/L of PM2.5 could cause a significantly high expression of NF-kB in A549 nuclear, but 40mg/L BLE could resist the activation of NF-kB stimulated by PM2.5. Conclusion BLE have the potential ability of DPPH free radical scavenging, anti-DNA strand breakage by H2O2-induced, anti-inhibitive effect on cell proliferation induced by the PM2.5 and anti-inflammation by decreasing the activation of NF-kB stimulated by PM 2.5. ? 2011 IEEE.