文献类型：期刊文献

中文题名：壳寡糖抗运动疲劳及对运动性免疫抑制的影响

英文题名：Effect of Chitosan Oligosaccharide on Exercise Anti-fatigue and Exercise-induced Immunosuppression

作者：尹雨芳[1];林强[1];曹建民[2];周海涛[3];王灿[2]

第一作者：尹雨芳

机构：[1]北京联合大学应用文理学院;[2]北京体育大学;[3]北京联合大学生物化学工程学院

第一机构：北京联合大学应用文理学院

年份：2016

卷号：22

期号：4

起止页码：146-149

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：北京市保健食品与生物活性物质重点实验室开放课题(ZK70201401)

语种：中文

中文关键词：壳寡糖;抗运动疲劳;运动性免疫抑制;炎症因子;Toll样受体4

外文关键词：chitosan oligosaccharide ; exercise-induced immunosuppression ; exercise-induced immunity ;inflammatory factors; Toll like receptor 4

摘要：目的:研究壳寡糖(chitosan oligosaccharide,CO)抗运动疲劳及对运动性免疫抑制的影响。方法:以大强度耐力训练大鼠为模型,55只SPF级Wistar 49 d龄雄性大鼠为对象,随机分为5组:静止组,运动组,运动+低、中、高剂量壳寡糖干预组,每组10只(剔除不符合实验要求的大鼠)。每天ig给药1次,壳寡糖干预组剂量分别为100,200,600 mg·kg^(-1),ig体积为5 m L·kg^(-1),其他组ig等体积生理盐水。42 d力竭游泳训练后,测试相关指标。结果:力竭游泳时间,静止组、运动组组间无显著差异,壳寡糖干预组较运动组均显著增加(P<0.01)。血清超氧化物歧化酶(SOD)活性,白细胞介素-10(IL^(-1)0)含量,运动组较静止组明显下降(P<0.01);壳寡糖干预组较运动组均显著升高(P<0.05,P<0.01)。丙二醛(MDA),核转录因子-κB(NF-κB),白细胞介素-6(IL-6),肿瘤坏死因子-α(TNF-α)含量,脾脏Toll样受体4(TLR4)表达率,运动组较静止组均显著升高(P<0.01),壳寡糖干预组较运动组均显著下降(P<0.05,P<0.01)。结论:壳寡糖可以有效缓解过度训练导致的机体内环境失衡,延缓和避免运动疲劳及运动性免疫抑制的发生与发展,且以高剂量组效果最为明显。
Objective： To study the effects of chitosan oligosaccharide （CO） on anti-exercise fatigue and exercise-induced immunosuppression. Method： To establish the model of high-intensity endurance training, 55 7-week-old male SPF Wistar rats were randomly divided into 5 groups, with 10 in each group （the rats that did not meet the requirements were removed） ： clam group （ C group） , exercise group （ E group） , exercise ＋ ig low-dose, middle-dose and high-dose chitosan oligosaccharide group （ CO ＋ E-L group, CO ＋ E-M group, CO ＋ E- H group）. Gavage was performed by using professional devices once a day. The rats in CO groups were garaged CO 100, 200, 600 mg ~ kg-1 with ig volume of 5 mL ~ kg-lrespectively. Rats in other groups were given saline of the same volume. After 42 days of exhaustive swimming training, measuring related indicators. Result： Swimming time in CO ＋ E groups were significantly longer than that in E group （ P 〈 0.01 ）. There were no difference between C and E groups. Serum superoxide dismutase （SOD） and interleukin-10 （IL-10） in E group were significantly lower than C group （ P 〈0.01 ） , at the same time, those in CO ＋ E groups were all higher than E groups （P 〈 0.05, P 〈 0.01 ）. Serum malondialdehyde （MDA） , nuclear factor-kappa B （NF-s：B） , interleukin-6 （IL-6） , tumor necrosis factor-α （TNF-α） and spleen Toll like receptor 4 （TLR4） expression ratio in E group were all significantly higher than C group （ P 〈 0. 01 ） , those in CO ＋ E groups were all lower than that in E group （P 〈0.05, P 〈 0.01 ）. Conclusion： The supplement of CO can effectively relieve the imbalance of internal environment caused by excessive training, avoid the occurrence and development of exercise fatigue and exercise immunosuppression and CO ＋ E-H group have a significant effect.