文献类型：期刊文献

中文题名：Development of a novel conductance-based technology for environmental bacterial sensing

英文题名：Development of a novel conductance-based technology for environmental bacterial sensing

作者：Shen FangXia[1];Tan MiaoMiao[2];Xu Hong[3];Xu ZhenCheng[4];Yao MaoSheng[1]

第一作者：Shen FangXia

通讯作者：Xu, ZC[1]

机构：[1]Peking Univ, State Key Joint Lab Environm Simulat & Pollut Con, Coll Environm Sci & Engn, Beijing 100871, Peoples R China;[2]Beijing Union Univ, Coll Mech & Elect Engn, Beijing 100020, Peoples R China;[3]Guangdong Women & Children Hosp, Dept Reprod Hlth, Guangzhou 510655, Guangdong, Peoples R China;[4]Minist Environm Protect, South China Inst Environm Sci, Guangzhou 510655, Guangdong, Peoples R China

第一机构：Peking Univ, State Key Joint Lab Environm Simulat & Pollut Con, Coll Environm Sci & Engn, Beijing 100871, Peoples R China

通讯机构：[1]corresponding author), Minist Environm Protect, South China Inst Environm Sci, Guangzhou 510655, Guangdong, Peoples R China.

年份：2013

卷号：58

期号：4

起止页码：440-448

中文期刊名：科学通报：英文版

外文期刊名：CHINESE SCIENCE BULLETIN

收录：;Scopus(收录号：2-s2.0-84874515326);WOS:【SCI-EXPANDED(收录号:WOS:000315445500002)】；PubMed;

基金：This work was supported by the National Natural Science Foundation of China (21277007), the National High Technology Research and Development Program of China (2008AA062503) and Environment and Health Joint Laboratory at South China Institute of Environmental Science (21111011101EHH(2011)-208).

语种：英文

中文关键词：技术开发;细菌检测;电导数;基础;Bacillus;细菌浓度;阻抗测量;环境

外文关键词：bacterial impedance; conductance; bioaerosol; bacterial concentrations

摘要：In this study, a simple impedance based technology for measuring bacterial concentrations was developed. The measurement system includes the signal amplification, copper probes and a sample loader. During the experiments, the conductance of Bacillus subtilis var niger, Pseudomonas fluorescens, and Escherichia coli were measured using the combination of a pre-amplifier and a lock-in amplifier. The conductance data were modeled verses the bacterial concentrations. Results indicated that the relationship between the conductance of bacterial suspensions and their concentrations follows a generic model: Y=C1+C2×e ( X/C3 ) , where Y is the conductance (S), X is the bacterial concentration (Number/mL: abbreviated to N/mL) for all species tested, and C1 3 are constants. Gram negative P. fluorescens and E. coli assumed similar conductance curves, which were flatter than that of gram positive B. subtilis var niger. For P. fluorescens and E. coli the culturing technique resulted in higher concentration levels (statistically significant) from 2 to 4 times that measured by the impedance based technology. For B. subtilis var niger, both methods resulted in similar concentration levels. These differences might be due to membrane types, initial culturability and the obtained conductance curves. The impedance based technology here was shown to obtain the bacterial concentration instantly, holding broad promise in realtime monitoring biological agents.
In this study, a simple impedance based technology for measuring bacterial concentrations was developed. The measurement system includes the signal amplification, copper probes and a sample loader. During the experiments, the conductance of Bacillus subtilis var niger, Pseudomonas fluorescens, and Escherichia coli were measured using the combination of a pre-amplifier and a lock-in amplifier. The conductance data were modeled verses the bacterial concentrations. Results indicated that the relationship between the conductance of bacterial suspensions and their concentrations follows a generic model: Y=C-1 + C-2 x e((-X/C3)), where Y is the conductance (S), X is the bacterial concentration (Number/mL: abbreviated to N/mL) for all species tested, and C1-3 are constants. Gram negative P. fluorescens and E. coli assumed similar conductance curves, which were flatter than that of gram positive B. subtilis var niger. For P. fluorescens and E. coli the culturing technique resulted in higher concentration levels (statistically significant) from 2 to 4 times that measured by the impedance based technology. For B. subtilis var niger, both methods resulted in similar concentration levels. These differences might be due to membrane types, initial culturability and the obtained conductance curves. The impedance based technology here was shown to obtain the bacterial concentration instantly, holding broad promise in realtime monitoring biological agents.