文献类型：期刊文献

英文题名：Rapid detection of genotoxicity employing an engineered high-sensitivity and tight SOS-response biosensor in Escherichia coli MG1655

作者：Yuan, Xin[1];Hao, Wenliang[2];Zhang, Chong[2,3,4,5];Ji, Yizhi[1];Wang, Yi[2,4,5]

第一作者：Yuan, Xin

通讯作者：Ji, YZ[1];Wang, Y[2]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]Tsinghua Univ, Inst Biochem Engn, Dept Chem Engn, MOE Key Lab Ind Biocatalysis, Beijing 100084, Peoples R China;[3]Tsinghua Univ, Ctr Synthet & Syst Biol, Beijing 100084, Peoples R China;[4]Beijing Key Lab Recombinant Prot & Biomfg, Beijing, Peoples R China;[5]State Key Lab Green Biomfg, Beijing, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]corresponding author), Tsinghua Univ, Inst Biochem Engn, Dept Chem Engn, MOE Key Lab Ind Biocatalysis, Beijing 100084, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2026

卷号：11

起止页码：117-126

外文期刊名：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY

收录：;Scopus(收录号：2-s2.0-105016407028);WOS:【SCI-EXPANDED(收录号:WOS:001665468000001)】；

基金：This work was supported by the National Key Research and Devel-opment Program of China (2022YFF0609100) , Guangdong S & T Pro-gram (2024B1111130002) and Research and Development Projects of Hebei Province (22375503D) .

语种：英文

外文关键词：Genotoxicity detection; Whole-cell biosensors; Environmental pollution; SOS response; Chemical monitoring

摘要：Industrial wastewater and pesticide residues contain numerous genotoxic compounds that pose serious health risks by causing DNA damage and potentially triggering carcinogenesis. Conventional analytical methods such as chromatography and mass spectrometry are limited by high costs, technical complexity, and lengthy procedures, making them unsuitable for rapid, large-scale screening. To address this need, we developed a fluorescence-based SOS-responsive whole-cell biosensor (SRWCB) by integrating the PsulA promoter with superfolder GFP (sfGFP). Through directed evolution of the lexA binding motif in PsulA, we obtained an optimized variant (P55) with significantly enhanced sensitivity-being 8.5 times more sensitive than the commonly used PumuDC system-while maintaining rapid response kinetics. Further engineering via antisense transcription improved biosensor tightness without compromising induction efficiency. The final construct (SRWCB-55-J23119) successfully detected genotoxicants in industrial wastewater, demonstrating its practical applicability. This study presents a sensitive, rapid, and robust biosensor platform for high-throughput environmental genotoxicity monitoring.