文献类型：期刊文献

英文题名：Novel Environmentally-Friendly Process for Selective Extraction and Enrichment of DHA/EPA-Containing Phospholipids from Krill Oil via Differential Temperature-Controlled Crystallization

作者：He, Yi[1];Zhang, Yu[1];Heng, Jiangying[1];Liu, Bo[1];Ma, Xuan[1];Jin, Jing[1];Yan, Wenjie[1,2];Wang, Feng[1]

通讯作者：Wang, F[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100101, Peoples R China;[2]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Food, Beijing 100101, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100101, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2025

卷号：14

期号：16

外文期刊名：FOODS

收录：;Scopus(收录号：2-s2.0-105014398831);WOS:【SCI-EXPANDED(收录号:WOS:001558249200001)】；

基金：This research was funded by the National Key Research and Development Program (2023YFF1103804), Major Science and Technology Project of Henan Province (No. 231100310200), and the Academic Research Projects of Beijing Union University (No. ZK20202516).

语种：英文

外文关键词：DHA/EPA-containing phospholipids; green extraction method; differential crystallization; response surface methodology; molecular species distribution; oxidative stability

摘要：This study presents a novel environmentally-friendly process for the selective extraction and enrichment of DHA/EPA-containing phospholipids (PL-DHA/EPA) from krill oil. The methodology leverages differential crystallization behavior between phospholipids and triacylglycerols in ethanolic solutions, exploiting their distinct freezing point thresholds to achieve precise fractionation. Response surface methodology optimization identified optimal extraction parameters: liquid-to-material ratio of 6:1 (v/w), freezing temperature of -20 degrees C, freezing duration of 25 h, and rotary evaporation temperature of 45 degrees C, yielding a final product with 39.40% PL-DHA/EPA content. Principal component analysis revealed substantial overlap in confidence ellipses among extraction methodologies, indicating effective preservation of core phospholipid signatures from the parent krill oil while maintaining critical structural characteristics and molecular species distribution. Comprehensive analysis of phospholipid fractions and heatmap analysis revealed distinctive molecular profiles compared to conventional organic solvent extraction, with selective enrichment of EPA-containing phospholipids, particularly PC-EPA and PI-EPA species. The green extraction method demonstrated comparable oxidative stability to conventional approaches, with superior protection against secondary oxidation as evidenced by significantly lower anisidine values. This sustainable approach achieves effective phospholipid enrichment while substantially reducing environmental impact through elimination of halogenated solvents, addressing the critical need for environmentally conscious technologies in marine lipid processing with potential applications in nutraceutical and functional food industries.