文献类型：期刊文献

中文题名：葛仙米对大鼠的润肠通便作用研究

英文题名：Effect of Nostoc sphaeroides on Laxative in Rats

作者：刘银路[1];毕萃萃[1];魏芬芬[1];陈盛[2];王玉兰[2];张波[1]

第一作者：刘银路

机构：[1]北京联合大学,功能食品科学技术研究院,生物活性物质与功能食品北京市重点实验室,北京100191;[2]湖南炎帝生物工程有限公司,湖南株洲412000

第一机构：北京联合大学生物化学工程学院功能食品科学技术研究院|北京联合大学应用文理学院

年份：2020

卷号：41

期号：16

起止页码：292-296

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2017】；

语种：中文

中文关键词：葛仙米;盐酸洛哌丁胺;便秘;润肠通便

外文关键词：Nostoc sphaeroides;loperamide hydrochloride;constipation;laxative

摘要：目的:研究葛仙米对便秘大鼠的润肠通便作用,并探讨其缓解便秘的相关机制。方法:随机将大鼠分为对照组、模型组、葛仙米组。模型组、葛仙米组灌胃葛仙米3周后再灌胃盐酸洛哌丁胺1周,对照组大鼠灌胃等量生理盐水。灌胃墨汁实验观察大鼠首粒排黑便时间。采用苏木精-伊红(Hematoxylin-eosin,HE)染色和阿利新蓝-过碘酸雪夫(Alcian blue-periodic acid Schiff,AB-PAS)染色,观察结肠结构以及粘液物质分泌情况。结果:与模型组大鼠比较,葛仙米组大鼠经葛仙米干预,极显著增加了便秘大鼠的粪便重量以及含水量(P<0.01);极显著缩短了首粒黑便产生时间(P<0.01);显著改善了结肠肌层和粘膜层结构,增加杯状细胞数量以及粘液物质含量(P<0.05)。结论:葛仙米通过显著增加大鼠大肠水分含量,加强肠蠕动,增加黏液分泌,对肠道组织保护具有促进作用,在本实验中具有较好的润肠通便作用。
Objective:To study the effect of Nostoc sphaeroides on laxative in constipation rats and explore the related mechanisms of relieving constipation. Methods:Rats were randomly divided into control group,model group and Nostoc sphaeroides group. The model group and the Nostoc sphaeroides group were intragastrically administered with Nostoc sphaeroides for 3 weeks and then administered with loperamide hydrochloride for 1 week. The rats in the control group were given the same amount of normal saline. The first black sputum time was observed by intragastric administration of the ink. Hematoxylin-eosin(hematoxylin-eosin,HE)staining and alixin blue-periodic acid Schiff(AB-PAS)staining were used to observe colonic structure and mucus secretion. Results:Compared with the model group rats,Nostoc sphaeroides group’s intervention with Nostoc sphaeroides extremely significantly increased the fecal weight and water content of constipated rats(P<0.01),and extremely significantly shortened the first black stool generation time(P<0.01). The structure of the colonic muscular and mucosal layers was improved,the number of goblet cells and the content of mucus increased significantly(P<0.05). Conclusion:Nostoc sphaeroides could significantly improve the intestinal water content,strengthen intestinal peristalsis,increase mucus secretion,protect intestinal tissues,and effectively improve constipation symptoms.