文献类型：期刊文献

英文题名：The effect of dense phase carbon dioxide on the conformation of hemoglobin

作者：Yan, Wenjie[1];Xie, Yangyang[1];Wang, Xiaoxi[1];Jia, Fei[2];Li, Xingmin[2]

第一作者：闫文杰

通讯作者：Li, XM[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China.

年份：2018

卷号：106

起止页码：885-891

外文期刊名：FOOD RESEARCH INTERNATIONAL

收录：;EI(收录号：20180604768677);Scopus(收录号：2-s2.0-85041473188);WOS:【SCI-EXPANDED(收录号:WOS:000429893100097)】；

基金：This study was funded by the Premium Funding Project for Academic Human Resources Development in Beijing Union University (grant no. BPHR2017C204), the National Key Research and Development Plan during the 13th five-year plan period (grant no. 2016YFD0401504).

语种：英文

外文关键词：Dense phase carbon dioxide; Hemoglobin; Circular dichroism; Fluorescence spectroscopy; Transmission electron microscope

摘要：Dense phase carbon dioxide (DPCD) sterilization is a non-thermal sterilization technology used to process heat sensitive foods. Although nutritional and sensorial quality of food is preserved while unwanted microbial activity is reduced during DPCD sterilization, the effect on protein structure remains unclear. In this work, the effect of DPCD on the higher order structure and fluorescence properties of Hemoglobin (Hb) was investigated. The different conditions assessed during DPCD processing included variation in pressure, pH and heating conditions. Results from this study showed an inversely proportional correlation between alpha-helical content of Hb and pressure. As the pressure was lowered, the levels of a-helical content increased. The increased levels of a helix correlated with a lower fluorescence intensity and a limited redshift in the fluorescence emission wavelength. TEM imaging showed that DPCD processing resulted in Hb with larger molecular diameters, which became smaller as the pressure increased. Interestingly, after 7-day storage at 4 degrees C, an increase in alpha-helical content was observed. Results from this work show that DPCD sterilization does impact the conformation of hemoglobin, with a notable impact on secondary and tertiary structure.