文献类型：期刊文献

中文题名：双歧杆菌二联制剂对小鼠抗氧化水平及肠道菌群的影响

英文题名：Effects of Bifidobacterium Double Preparation on Antioxidant Levels and Intestinal Flora of Mice

作者：王韵婷[1];刘倩[1];郭子晨[1];孙雅煊[1];霍清[1];王伶毓[2];赵江燕[2]

第一作者：王韵婷

机构：[1]北京联合大学生物化学工程学院,北京100023;[2]北京联合大学应用文理学院保健食品功能检测中心,北京100191

第一机构：北京联合大学生物化学工程学院

年份：2022

卷号：47

期号：5

起止页码：13-20

中文期刊名：食品科技

外文期刊名：Food Science and Technology

收录：北大核心:【北大核心2020】；

基金：国家自然科学基金项目(11975048);北京联合大学研究生科研创新资助项目(YZ2020K001);北京联合大学开放课题(SWHX202103);北京联合大学科研资助项目(XP202007)。

语种：中文

中文关键词：长双歧杆菌BB536;乳双歧杆菌HN019;肠道菌群;短链脂肪酸;高通量测序

外文关键词：Bifidobacterium longum BB536;Bifidobacterium lactis HN019;intestinal flora;short chain fatty acid;high-throughput sequencing

摘要：目的:旨在探究长双歧杆菌BB536联合乳双歧杆菌HN019益生菌制剂对小鼠抗氧化水平及肠道菌群的影响,为益生菌制剂的开发利用提供依据。方法:将48只小鼠随机分为空白(Control)和低(L)、中(M)和高剂量(H)组,分别灌胃生理盐水和不同剂量的双歧杆菌二联制剂,连续30 d。给药的第0、30天,采集小鼠粪便样品进行细菌计数,30 d采集小鼠粪便进行16S rDNA高通量测序以及短链脂肪酸测定,并测定小肠、肝脏组织中总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX)、谷胱甘肽(GSH)和丙二醛(MDA)的水平。结果:与Control组相比,双歧杆菌二联制剂灌胃对小鼠的摄食、体重和脏器系数等无显著影响。小肠组织中H组T-SOD活力、GSH水平极显著升高(P<0.01);M、H组均能极显著升高GSH-Px水平(P<0.01),极显著降低MDA水平(P<0.01)。肝脏组织中,与Control组相比,M组GSH、GSH-Px显著升高(P<0.05),在H组中极显著提高(P<0.01)。在肝脏中,M、H组与Control相比,T-SOD活力极显著提高(P<0.01),MDA水平极显著下降(P<0.01)。灌胃30 d后,与Control组相比,各剂量组肠杆菌和肠球菌含量无显著差异,M组的乳杆菌和双歧杆菌含量显著增加(P<0.05),H组的乳杆菌和双歧杆菌含量极显著增加(P<0.01)。16S rDNA高通量测序结果表明,与Control组相比,L组Lachnospiraceae_NK4A136.group和Odoribacter丰度显著升高(P<0.05),M组罗氏菌属丰度显著升高(P<0.05),Mucispirillum和Oscillibacter丰度显著降低(P<0.05);H组中粪杆菌属丰度显著增加(P<0.05),双歧杆菌属丰度极显著增加(P<0.01)。灌胃双歧杆菌二联制剂可有效提高乙酸(P<0.01)、丙酸(P<0.01)和丁酸(P<0.05)含量。结论:综上,益生菌长双歧杆菌BB536联合乳双歧杆菌HN019益生菌制剂能够有效增加GSH水平及T-SOD、GSH-PX活力,降低MDA水平,抑制Mucispirillum和颤杆菌属有害菌的增殖,促进有益菌Lachnospirace_Nk4A136_group、Odoribacter、罗氏菌属、双歧杆菌属和粪杆菌属的生长,提高乙酸、丙酸和丁酸3种短链脂肪酸含量,增强机体抗氧化能力,改善肠道微环境。
Objective:This study was conducted to test the effects of Bifidobacterium longum BB536 combined with Bifidobacterium lactis HN019 on antioxidant levels and the intestinal flora of mice,in order to supply scientific basis for further development and utilization of probiotics compound preparation.Methods:Forty-eight mice were randomly divided into control,low-dose(L),medium-dose(M)and high-dose(H)groups,and were respectively administered with normal saline and different doses of Bifidobacterium double preparation for 30 consecutive days.On the 0th and 30th day of administration,mice feces were collected for viable bacteria count.Mice feces were collected for 16S rDNA high-throughput sequencing and short-chain fatty acid determination.The levels of SOD,MDA,GSH and GSH-PX in small intestine and liver tissues were determined.Results:Compared with the control group,there was no significant difference in food intake,body weight and organ coefficient in each dose group.In the small intestine tissue,the activity of T-SOD and the level of GSH in the H group were significantly increased(P<0.01),the level of GSH-PX in the M and H group were significantly increased(P<0.01)and the level of MDA was significantly decreased(P<0.01).In the liver tissue,compared with the control group,the level of GSH and GSH-PX were significantly increased in the M group(P<0.05)and extremely increased in the H group(P<0.01),the activity of T-SOD was significantly increased(P<0.01)and the level of MDA was significantly decreased(P<0.01)in the M and H group.After intragastric administration for 30 days,compared with the control group,there was no significant difference in the content of Enterobacter and Enterococcus in each dose group,the contents of Lactobacillus and Bifidobacterium in the M group were increased significantly(P<0.05),the contents of Lactobacillus and Bifidobacterium were increased significantly at the H group(P<0.01).The 16S rDNA high-throughput sequencing results showed that,compared with the control group,the abundance of Lachnospiraceae_NK4A136_group and Odoribacter were significantly increased in the L group(P<0.05);and the abundance of Roseburia was significantly increased(P<0.05),and the abundance of Mucispirillum and Oscillibacter were significantly decreased in the M group(P<0.05).In the H group,the abundance of Faecalibacterium was significantly increased(P<0.05)and the abundance of Bifidobacterium increased significantly(P<0.01).Gavage of Bifidobacterium double preparation can effectively increase the content of acetic acid(P<0.01),propionic acid(P<0.01)and butyric acid(P<0.05).Conclusion:Bifidobacterium longum BB536 combined with Bifidobacterium lactis HN019 probiotic preparation,which can change the structure of the intestinal flora,inhibit the proliferation of Mucispirillum and Oscillatoria harmful bacteria,and promote the growth of Lachnospirace_Nk4A136_group,Odoribacter,Rosella,Bifidobacterium and Faebacterium,increase the content of three short-chain fatty acids,including acetic acid,propionic acid and butyric acid,enhance the antioxidant capacity and improve the intestinal microenvironment.