文献类型：期刊文献

英文题名：Protective Effect of Zeaxanthin against Tunicamycin-induced Cell Damage in SH-SY5Y Cell

作者：Wei, Jun[1];Zhao, Fanfan[1];Shang, YingHui[1];Liu, Xinjun[1];Huang, Hanchang[1];Lao, Fengxue[1]

通讯作者：Lao, FX[1]

机构：[1]Beijing Union Univ, Dept Biochem & Engn, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Dept Biochem & Engn, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;[114172]北京联合大学应用文理学院;

年份：2018

卷号：24

期号：6

起止页码：1101-1109

外文期刊名：FOOD SCIENCE AND TECHNOLOGY RESEARCH

收录：;EI(收录号：20191406725927);Scopus(收录号：2-s2.0-85063659004);WOS:【SCI-EXPANDED(收录号:WOS:000453341300017)】；

基金：We are grateful to Professor Yinghui Shang and Hanchang Huang and of Beijing Union University for excellent technical assistance. This study was supported by National Natural Science Foundation of China (31471587).

语种：英文

外文关键词：zeaxanthin; endoplasmic reticulum stress; tunicamycin; SH-SY5Y cells; Alzheimer's disease

摘要：Endoplasmic reticulum (ER) stress and the resulting neuronal cell damage have been implicated in the development and progression of Alzheimer's disease. Tunicamycin (TM) induces ER stress in vitro, and the protective effects of the carotenoid zeaxanthin against the effects of TM have recently been studied. Zeaxanthin has been shown to hold several beneficial health properties in human studies. The protective role of zeaxanthin against the toxic effects of TM has recently been studied for the first time. The present study investigated whether zeaxanthin protects SH-SY5Y cells against TM-induced cell damage in vitro. Both pretreatment and post-treatment with zeaxanthin increased cell viability and suppressed lactate dehydrogenase release compared to levels in controls. Further, we found that zeaxanthin considerably ameliorated TM-induced cell damage by protecting the integrity of the mitochondrial membrane decreasing caspase-3 activity, and by reducing the apoptosis rate as well as the expression of the ER stress biomarker GRP78. Modulation of GRP78 may be one of the mechanisms by which zeaxanthin affects cell viability. Our results indicate a potential application of zeaxanthin in the development of new therapeutic drugs for the treatment of Alzheimer's disease.