文献类型：期刊文献

中文题名：基于正交设计的离子选择性微电极硅烷化试验研究

英文题名：Research on Ion Selective Microelectrode Silanization Experiment Based on Orthogonal Design

作者：薛琳[1,2];王文博[1];王子洋[1];王忠义[1];黄岚[1]

第一作者：薛琳

机构：[1]中国农业大学信息与电气工程学院,北京100083;[2]北京联合大学信息学院,北京100101

第一机构：中国农业大学信息与电气工程学院,北京100083

年份：2016

卷号：0

期号：4

起止页码：32-37

中文期刊名：现代科学仪器

外文期刊名：Modern Scientific Instruments

收录：CSTPCD

基金：教育部博士点基金（20130008110035）;国家自然科学基金（61571443）.

语种：中文

中文关键词：离子选择性微电极;硅烷化;正交设计;定量

外文关键词：Ion selective microelectrode; Silanization; Orthogonal design; Quantify

摘要：离子选择性微电极是一种电化学传感器，用于测量被测样品的离子浓度和离子流速。对离子选择性微电极进行硅烷化处理可以延长离子选择性微电极的使用寿命。目前，受限于试验条件的不同，微电极的硅烷化处理重复性很差，造成不必要的浪费，因此对于影响离子选择性微电极一致性和重复性的硅烷化过程还需要进一步规范和量化。本文采用正交试验设计方法，定量研究了硅烷试剂用量、硅烷温度、硅烷时间对硅烷化效果的影响程度。结果表明当硅烷化器皿容量为125mL、环境温度25℃、湿度低于50％时，使用二甲基二氯硅烷作为硅烷试剂对无导液丝的薄壁玻璃毛细管硅烷化时，硅烷试剂用量20μL，硅烷化温度150℃，硅烷化时间30-45min的试验条件下微电极的硅烷化效果最佳。
Ion selective microelectrode is an electrochemical sensor used for measuring the ion concentration and ion flux of samples. The surface of ion selective microelectrode must be silanized to prolong the service life of ion selective microelectrode. Now in the experiments, it is hard to repeat to get the same silanization results, because of being limited by the different experimental conditions. Thus, silanization process which affects the consistency and repeatability of ion selective microelectrode needs to further standardize and quantified. This paper studied the effects of dosage of silane reagent, reaction temperature, and reaction time on the silanization results based on orthogonal design quantitatively Experimental results showed that the best parameters of dimethyl dichlorosilane reagent, silanization temperature and silanization time were 20 μL, 150 ℃ and 30-45 min to achieve the best results of microelectrode silanization in a 125-mL airtight container when environment temperature was 25 ℃ and humidity was below 50% using borosilicate thin-wall glass tubes without filament