文献类型：期刊文献

中文题名：豆甾醇分子印迹聚合物的合成及性能研究

英文题名：SYNTHESIS AND PROPERTY OF STIGMASTEROL MOLECULAR IMPRINTING POLYMER

作者：韩永萍[1,2];何绪文[1];林强[2]

第一作者：韩永萍

机构：[1]中国矿业大学化学与环境工程学院,北京100083;[2]北京联合大学生物化学工程学院,北京100023

第一机构：中国矿业大学化学与环境工程学院,北京100083

年份：2008

卷号：24

期号：5

起止页码：451-459

中文期刊名：离子交换与吸附

外文期刊名：Ion Exchange and Adsorption

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：北京市教委支持项目(KM200511417005)

语种：中文

中文关键词：分子印迹聚合物;豆甾醇;吸附容量;特异吸附性能

外文关键词：Molecular imprinting polymer; Stigmasterot; Adsorptive capacity; Specific recognition and binding performance.

摘要：以豆甾醇(stig.)为印迹分子、甲基丙烯酸甲酯(MMA)为功能单体、二乙烯基苯(DVB)为交联剂、碳酸钙及十二烷基硫酸钠(SDS)混合物为分散剂、过硫酸钠为引发剂、极性较低的甲醇(tolu.)为致孔剂,采用悬浮聚合法合成豆甾醇分子印迹聚合物。并对试验合成的分子印迹聚合物的形貌、粒径及选择吸附性能分别采用高效液相、电镜和光学显微镜进行分析和评价。试验结果表明,最佳配方为3gstig.:40mL MMA:15mL DVB:4gCaCO3+2g SDS:0.4mLNa2S2O8:14mL tolu.,其中引发剂过硫酸钠应分多次加入,在聚合反应初期采用高速搅拌,随着聚合反应的进行搅拌速度应逐渐减小。该条件下合成的分子印迹聚合物平均粒径小于100μm、分布较窄,对豆甾醇表现出快速和稳定的特异吸附性能,其分离因子超过1.65,吸附容量约为1.2mg/g。
The molecular imprinting polymeric microspheres （MIPs） of stignmasterol was synthesized by suspension polymerization, utilizing stignmasterol （stig.） as template molecule, methyl methacrylate （MMA） as functional monomer, divinyl benzene （DVB） as cross-linker agent, CaCO3 and SDS as dispersant, Na2S2O8 as initiator and lowpolar solvents （toluene） as porogenic solvents. The morphology, averages size and selectivity for stignmasterol of the synthesized polymers were respectively examined and evaluated by HPLC, SEM and Photon Correlation Spectroscopy. The experiment results showed that the optimal condition was 3g stig. ：40mL MMA： 15mL DVB ：4g CaCO3＋2g SDS ： 14mL tolu.. During polymerization initiator Na2S2O8 should be added in more batches and in the early period a high speed string was applied, with the polymerization the string rate was reduced. The prepared MIPs, which average diameters were below 100μm and narrow size distribution, could effectively separate stignmasterol from structural analogues β -stiosterol with above 1.65 separate factor and about 1.2mg/g adsorptive capacity for stigmasterol.