文献类型：期刊文献

中文题名：林蛙油对运动训练大鼠睾酮含量、物质代谢及抗运动疲劳能力的影响

英文题名：Effect of Oviductus Ranae on Testosterone Content,Substance Metabolism and Exercise Capacity in Rats Receiving Exercise Training

作者：娄春善[1];曹建民[2];郭娴[2];周海涛[3];商晓绪[4]

第一作者：娄春善

机构：[1]河北师范大学;[2]北京体育大学;[3]北京联合大学生物化学工程学院;[4]北京市朝阳区教育研究中心

第一机构：河北师范大学,石家庄050024

年份：2014

卷号：20

期号：20

起止页码：150-154

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2011】；

基金：河北省教育厅重点资助项目(ZD20131100)

语种：中文

中文关键词：林蛙油;睾酮;抗疲劳

外文关键词：Oviductus Ranae; testosterone; anti-fatigue

摘要：目的:研究林蛙油对运动训练大鼠睾酮含量、物质代谢及抗运动疲劳能力的影响。方法:以大强度耐力训练大鼠为模型,55只42 d龄雄性Wistar大鼠为对象,随机分为5组:静止对照组(C组)、运动对照组(M组)、运动+低剂量林蛙油组(OML组),运动+中剂量林蛙油组(OMM组),运动+高剂量林蛙油组(OMH组),每组10只(剔除不符合实验要求的大鼠5只)。每天灌胃(ig)1次,林蛙油组剂量分为0.5,1.0,3.0 g·kg-1,灌胃体积为5 mL·kg-1,C,M组ig等量生理盐水。42 d力竭游泳训练后,测定体重、力竭游泳时间及血睾酮等生化指标。结果:体重:运动对照组小于静止对照组(P<0.05);林蛙油各组大于运动对照组(P<0.05),组间无显著差异。力竭游泳时间:运动对照组与静止对照组无明显差异;林蛙油各组明显长于运动对照组(P<0.01),且随剂量增大而延长。血清睾酮水平:运动对照组(P<0.01)低于静止对照组;林蛙油各组分别为(4.96±1.60),(5.19±1.60),(5.35±1.66)nmol·L-1,高于运动对照组(P<0.01),组间无显著差异。血清皮质酮水平:各组间均无显著差异。血清睾酮与皮质酮比值变化与睾酮变化较为一致。肝、肌糖原水平:运动对照组(P<0.01)低于静止对照组;林蛙油各组高于运动对照组(分别为P<0.05,P<0.01),组间无显著差异。促黄体生成素、促卵泡激素各组间无显著差异。血尿素水平:运动对照组(P<0.01)高于安静对照组;林蛙油各组低于运动对照组(P<0.01),且组间无显著差异。血红蛋白水平:运动对照组(P<0.01)低于静止对照组;林蛙油各组高于运动对照组(P<0.01),且组间无显著差异。结论:林蛙油可以减轻血睾酮受高强度运动量的影响,并维持在正常生理水平;可以促进蛋白质合成,抑制氨基酸和蛋白质分解,提高运动训练大鼠血红蛋白含量和糖原的储备。
Objective：To study the effects of Oviductus Ranae on the content of testosterone,substance metabolism and anti-fatigue ability of rats after exercise.Method：By using the model of high-intensity endurance training,fifty-five male Wistar rats were randomly divided into 5 groups,with 10 in each group.The rats which did not meet the requirement were removed：still in control group （C group）,motion control group （M group）,exercise ＋ ig low-dose Oviductus Ranae group （OML group）,exercise ＋ ig middle-dose Oviductus Ranae group （OMM group）,and exercise ＋ ig high-dose Oviductus Ranae group （OMH group）.Gavage was performed using professional device once a day.The rats in Oviductus Ranae groups were gavaged with 0.5,1.0,3.0 g ·kg-1 with ig volume of 5 mL ·kg-1 The rats in C and M groups were given saline of same volume.After 42 days of exhaustive swimming training,body weight,swimming time and serum testosterone and other biochemical markers were measured.Result：The body weights of the rats in M groups were lower than those in C group （P 〈 0.05）,and those in all doses of Oviductus Ranae groups were higher than in M group （P 〈 0.05） which showed no differences between groups.Swimming time in all doses of Oviductus Ranae groups was longer than M group （P 〈0.01） with a dose-effect relationship,But there were no differences between C and M groups.Serum testosterone in M group was lower than C group （P 〈 0.01）,in the same time,serum testosterone was higher in all doses of OviductusRanaegroupsthanM group [OML：（4.96±1.60） nmol·L^-1; OMM：（5.19±1.60） nmol·L^-1;OMH：（5.35 ± 1.66） nmol ·L^-1,P 〈 0.01].The serum corticosterone levels in each group showed no significant differences.Changes in the ratio of serum testosterone/corticosterone were more consistent with testosterone changes among the groups.Liver glycogen and muscle glycogen （P 〈 0.01） in M group were lower than in C group,and in all doses of Oviductus Ranae groups were higher than in M group （liver glycogen,P 〈0.05; muscle glycogen,P 〈 0.01）.There were no differences in luteinizing hormone and follicle-stimulating hormone between groups.The serum urea nitrogen in M group was higher than C group （P 〈 0.01）,and in all doses of Oviductus Ranae groups was lower than the M group （P 〈 0.01）.Hemoglobin in M group was lower than C group （P 〈0.01）,and in all doses of Oviductus Ranae groups were higher than in M group （P 〈0.01）.Conclusion：Oviductus Ranae can alleviate the impact of high-intensity exercise on serum testosterone,and maintained it at normal physiological levels; it can also promote protein synthesis,inhibit degradation of amino acid and protein,and increase hemoglobin and glycogen reserves in rats exercise training.