详细信息
文献类型:期刊文献
中文题名:一种测定枸杞鲜果中总类胡萝卜素含量的方法
英文题名:A method to determine total carotenoid amount from fresh wolfberry fruit
作者:蔡靳[1];惠伯棣[2];蒋继志[1];吴真真[1];宫平[2];关欣[2]
第一作者:蔡靳
机构:[1]河北大学生命科学院;[2]北京联合大学应用文理学院食品科学系
第一机构:河北大学生命科学院,保定071002
年份:2013
期号:2
起止页码:250-256
中文期刊名:中国食品添加剂
外文期刊名:China Food Additives
收录:CSTPCD;;北大核心:【北大核心2011】;
语种:中文
中文关键词:玉米黄素;玉米黄素酯;癸酸;反胶束;超分子溶液;枸杞
外文关键词:Zeaxanthin ; Zeaxanthin ester ; Decanoic acid; Inverted - micelle ; Supramolecular solvent ; Wolfberry
摘要:研究的目的是建立一种应用四氢呋喃-癸酸反胶束溶液作为样品萃取溶媒测定枸杞鲜果中总类胡萝卜素含量的方法。在实验中,比较了四氢呋喃、癸酸和四氢呋喃-癸酸反胶束溶液对枸杞鲜果中总类胡萝卜素的萃取效率,测定了四氢呋喃-癸酸反胶束溶液萃取玉米黄素酯的重复性和加样回收率,确定了样品萃取方法。同时测定玉米黄素酯在癸酸反胶束溶液中的吸光系数,建立了以四氢呋喃-癸酸反胶束溶液为样品液在UV-VIS上检测枸杞鲜果中总类胡萝卜素含量的方法。实验结果表明:四氢呋喃-癸酸反胶束溶液对枸杞鲜果样品具有良好的萃取效率和重复性(CV=6.24%)以及94.7%的玉米黄素棕榈酸双酯加样回收率。玉米黄素棕榈酸双酯在四氢呋喃-癸酸反胶束溶液中的吸光系数(A11%cm)为1221。可用来计算样品中的总类胡萝卜素含量。
This study aims at establishing a determination method of total carotenoid amount (TCA) from fresh wolfber- ry fruit. Tetrahydrofuran (THF) -decanoic acid inverted -micelle solution (DAIMS) was used as extracting medi- um. In the experiment, total carotenoid extracting efficiency comparison was made between THF, decanoic acid and THF - DAIMS. The reproducibility and recovery of zeaxanthin ester (ZE) by THF - DAIMS were determined. The ab- sorption coefficient ( AllUre ) of ZE was measured in THF - DAIMS. Data from those experiments suggested that the THF - DAIMS had a good extracting efficiency, reproducibility ( CV = 6. 24% ) and 94. 7% recovery of ZE. ZE ( AllUre ) was 1221 in THF - DAIMS which can be used in the calculation of TCA from the sample.
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