文献类型：期刊文献

英文题名：Chemical characterization of a PD-1/PD-L1 inhibitory activity fraction of the ethanol extract from Gymnadenia conopsea

作者：Lin, Peng-Cheng[1];Wang, Xin[2];Zhong, Xiang-Jian[2];Zhou, Na[2];Wu, Lei[1];Li, Jin-Jie[2];Hu, Yang-Tao[3];Shang, Xiao-Ya[2]

第一作者：Lin, Peng-Cheng

通讯作者：Shang, XY[1];Hu, YT[2]

机构：[1]Qinghai Nationalities Univ, Coll Pharmaceut Sci, Xining 810000, Peoples R China;[2]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China;[3]Chinese Peoples Liberat Army Joint Logist Support, Hosp 908, Nanchang 330047, Jiangxi, Peoples R China

第一机构：Qinghai Nationalities Univ, Coll Pharmaceut Sci, Xining 810000, Peoples R China

通讯机构：[1]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China;[2]corresponding author), Chinese Peoples Liberat Army Joint Logist Support, Hosp 908, Nanchang 330047, Jiangxi, Peoples R China.|[114172]北京联合大学应用文理学院;[11417]北京联合大学;[1141726]北京联合大学生物化学工程学院;

年份：2021

卷号：23

期号：3

起止页码：235-249

外文期刊名：JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000596184200001)】；

基金：This work financially supported by the grants from the National Natural Sciences Foundation of China (NNSFC; Grant No. 81760783); the Innovation Platform for the Development and Construction of Special Project of Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province (2020-ZJ-Y20) and Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University (SWHX202005); the Scientific Research Common Program of Beijing Municipal Commission of Education (Grant No. KM201911417014).

语种：英文

外文关键词：Gymnadenia conopsea; PD-1/PD-L1 inhibitory activity fraction; UPLC-HRMS/MS; succinic acid ester glycosides

摘要：Searching for PD-1/PD-L1 inhibitor from medicinal plants has become a potential method to discover small molecular cancer immunotherapy drugs. Using PD-1/PD-L1 inhibitory activity assay in vitro, a bioactive fraction was obtained from the ethanol extract of Gymnadenia conopsea. A sensitive UPLC-HRMS/MS method was established for the rapid screening and identification of compositions from bioactive fraction. Based on the characteristic fragmentation patterns of standards analysis and extracted ion chromatogram (EIC) method, 46 compounds were rapidly screened and identified (including 35 succinic acid ester glycosides and 11 other compounds), among which 17 compounds were tentatively identified as new compounds. [GRAPHICS] .