文献类型：期刊文献

中文题名：淡豆豉中多糖的分离纯化及结构特征研究

英文题名：Isolation,Purification and Structure Chareacterization of Polysaccharides from Semen Sojae Preparatum

作者：张静[1,2];林强[1];田平芳[2];葛喜珍[1];薛文静[1]

第一作者：张静

机构：[1]北京联合大学生物化学工程学院;[2]北京化工大学生命科学与技术学院

第一机构：北京联合大学生物化学工程学院

年份：2008

卷号：49

期号：10

起止页码：618-620

中文期刊名：化学世界

外文期刊名：Chemical World

收录：北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：北京市教育委员会科技发展计划资助项目(KM200611417013)

语种：中文

中文关键词：淡豆豉;多糖;凝胶层析;凝胶渗透色谱;结构特征

外文关键词：Seine Sojae Preparatum; polysaccharides; gel column chromatography, GPC; structure characterization

摘要：以中药淡豆豉为原料,采用超声法提取淡豆鼓多糖,硫酸-苯酚法检测总多糖含量。通过透析、醇沉、酶解蛋白、H2O2脱色,并经Sephadex G-100凝胶柱层析进一步纯化,得到多糖组分SPSS和SPSSII。利用凝胶渗透色谱(GPC)对其进行相对分子质量分析,红外光谱检测主要结构特征。结果表明,淡豆豉中总多糖含量为0.91%,SPSSI和SPSSII为均一性多糖,相对分子质量分别约为9500和7000,其结构具有多糖类物质的典型特征。
Polysaccharides were extracted using ultrasonic from the raw material of Semen Sojae Preparatum. The total content of polysaccharides was detected with a UV spectrophotometer after eolcration by sulphuric acid and phenylic acid. After dialyzing, depositing with alcohol, enzymolysising the protein with pepsin and decolring by H2O2 the polysaccharides were further purified by Sephadex G-100 column chromatography,and then SPSS Ⅰ and SPSS Ⅱ were obtained. The relative molecular mass of SPSS Ⅰ and SPSS Ⅱ was analyzed respectively by the method of gel-permeation chromatography（GPC） ,and their structure was characterized by IR spectroscopy. The experiments indicated that the total content of polysaccharides was 0.91% in Semen Sojae Preparatum Both SPSSI and SPSSII were uniform polysaccharide,whose relative molecular mass were about 9500 and 7000 respectively IR spectra showed that both of them had the typical stucture of polysaccharide.