文献类型：期刊文献

英文题名：Optimization of Capillary Electrophoresis Separation Conditions of Chlorpromazine, Promethazine and Their Main Metabolites by RSM

作者：Li, Chungeng[1];Cheng, Yanling[1,2];Zhou, Kaowen[1,2]

第一作者：Li, Chungeng

通讯作者：Cheng, YL[1];Zhou, KW[1];Cheng, YL[2];Zhou, KW[2]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2021

卷号：16

期号：11

起止页码：1-13

外文期刊名：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

收录：;Scopus(收录号：2-s2.0-85117899079);WOS:【SCI-EXPANDED(收录号:WOS:000718325900021)】；

基金：This work was supported by Beijing Natural Science Foundation of China (Grant No.2152013).

语种：英文

外文关键词：Capillary electrophoresis; Chlorpromazine; Promethazine; Metabolite; Response surface methodology

摘要：It is difficult to separate multiple phenothiazines with similar structures by capillary electrophoresis. However, under the experimental conditions optimized by response surface methodology (RSM), ten similar molecules of chlorpromazine, promethazine and their metabolites can be well separated in our experiment. Among them, the resolution of two difficult-to-separate molecules (chlorpromazine sulfoxide and hydroxychlorpromazine) is 1.39 (incomplete separation) under single factor experimental conditions and 1.51 (complete separation) under optimized experimental conditions. The optimum values of pH of phosphate buffer solution (PBS), concentration of NaCl in PBS, concentration of tween 40 in PBS and separation voltage were 6.75, 49.06 mmol/L, 26.58 mmol/L and 16.86 kV, respectively. All of the ten phenothiazines have good linear relationship. The recoveries of standard addition for dog urine samples without pretreatment were 87.3%-112.6%.