文献类型：期刊文献

英文题名：Study on Degradation Behavior and Biocompatibility of Polymethyl Methacrylate/Mineralized Collagen/Mg-Ca Alloy Composite Material

作者：Shen, Yuan-Xin[1];Sun, Xi-Rao[1];Wang, Cheng-Yue[1];Yang, Jing-Xin[2];Bao, Jia-Xin[1]

第一作者：Shen, Yuan-Xin

通讯作者：Wang, CY[1];Yang, JX[2]

机构：[1]JinZhou Med Univ, Dept Prosthodont, Affiliate Stornatol Hosp, Jinzhou 121000, Liaoning, Peoples R China;[2]Beijing Union Univ, Coll Robot, Beijing 100027, Peoples R China

第一机构：JinZhou Med Univ, Dept Prosthodont, Affiliate Stornatol Hosp, Jinzhou 121000, Liaoning, Peoples R China

通讯机构：[1]corresponding author), JinZhou Med Univ, Dept Prosthodont, Affiliate Stornatol Hosp, Jinzhou 121000, Liaoning, Peoples R China;[2]corresponding author), Beijing Union Univ, Coll Robot, Beijing 100027, Peoples R China.|[1141739]北京联合大学机器人学院;[11417]北京联合大学;

年份：2020

卷号：10

期号：2

起止页码：139-150

外文期刊名：JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000549441100001)】；

基金：This research was supported by The Science and Technology Fund of Liaoning Province (No. 20180530071), Liaoning Provincial Natural Fund Subsidy Program (No. 2019-MS-141), Beijing excellent talent project, (No. 2018000020124G073).

语种：英文

外文关键词：Polymethyl Methacrylate Bone Cement; Mineralized Collagen; Ma-Ca Alloy; Degradation Behavior; Biocompatibility

摘要：In this study, we composited mineralized collagen and magnesium-calcium alloy by freeze-drying, followed by dip-coating PMMA bone cement to enhance the composite of mineralized collagen and magnesium-calcium alloy. In vitro degradation test was performed to observe the pH and weight loss of the material. The contact angle test was used to detect the hydrophilicity of the material. Subsequently, MC3T3-E1 were used to assess cell biocompatibility in vitro by cell adhesion, cytotoxicity, alkaline phosphatase, alizarin red staining, and cytoskeleton. The results showed that the pH changes of the PMMA/NHAC/Mg-Ca was slower than that of the Mg-Ca, and the weight loss rate at 7 d and 14 d were lower than that of the Mg-Ca (P < 0.05) in degradation test. Wettability experiment showed that PMMA/NHAC/Mg-Ca was a hydrophilic material and Mg-Ca was a hydrophobic material (P < 0.05). In vitro cell experiments, the PMMA/NHAC/Mg-Ca had more cell adhesion than Mg-Ca and more synapses were connected to others. In the cytotoxicity experiment, the cell proliferation lever of PMMA/NHAC/Mg-Ca was higher than that of Mg-Ca at each time point (P < 0.05). In the 7 d alkaline phosphatase experiment, the PMMA/NHAC/Mg-Ca showed higher ALP activity than the Mg-Ca (P < 0.05), and in the alizarin red experiment at 14 d and 28 d, there were more obvious calcified nodules and mineralized area. After 1 day of culture in the PMMA/NHAC/Mg-Ca extract, the cells showed a clearer and more complete cytoskeletal structure and better cell morphology. In conclusion, PMMA/NHAC/Mg-Ca orthopedic implants had a better hydrophilicity, cytotoxicity and osteogenic ability, besides with a slower rate of degradation, and could be implanted in animals for further research, which were expected to be used for the repair of clinical bone defects.