文献类型：期刊文献

中文题名：碱性果胶酶诱导植物抗病的研究

英文题名：Studies on inducing plant disease resistance by alkaline pectinase

作者：李祖明[1];白志辉[2];张洪勋[2];李鸿玉[1];荣瑞芬[1];叶磊[1]

机构：[1]北京联合大学师范学院,北京100011;[2]中国科学院生态环境研究中心,北京100085

第一机构：北京联合大学师范学院

年份：2008

卷号：28

期号：9

起止页码：5-8

中文期刊名：中国植保导刊

外文期刊名：China Plant Protection

收录：北大核心:【北大核心2004】；

基金：国家自然科学基金(30600082);北京市优秀人才培养资助项目(20061D0502200295);北京市教委资助项目(KM200811417006)

语种：中文

中文关键词：吉氏芽孢杆菌;克劳氏芽孢杆菌;碱性果胶酶;植物抗病性;黄瓜黑星病

外文关键词：Bacillus gibsonii ; Bacillus clausii; alkaline pectinase; plant disease resistance ; cucumber scab

摘要：对碱性果胶酶诱导黄瓜抗病作用进行了研究。结果表明,菌株吉氏芽孢杆菌S-2(B.gibsoniiS-2)和克劳氏芽孢杆菌S-4(B.clausiiS-4)发酵生产的碱性果胶酶对黄瓜黄化苗具有诱导抗病作用。在不同酶活的处理中,S-2(200u/ml)和S-4(20u/ml)碱性果胶酶对黄瓜叶和茎上黑星病的病情指数降低最多,其诱导防病效果分别达到73.7%,80.0%和80.6%,86.6%。pH变化对碱性果胶酶的诱导抗病作用影响显著。碱性果胶酶S-2(200u/ml)和S-4(20u/ml)在pH8.0时诱导防病效果较好,它们对黄瓜叶和茎上黑星病的诱导防病效果分别达到64.8%,78.5%和75.0%,87.8%。
Efficiency of induced resistance to cucumber scab by alkaline pectinases was studied. The results indicated that when the alkaline pectinases from Bacillus gibsonii S-2 and Bacillus clausii S-4 were separately sprayed on cucumber seedlings, plant disease resistance was induced. The alkaline pectinase might be used in green food and organic food production. Among the treatments with different alkaline pectinase activity, the alkaline pectinases from both B. gibsonii S-2 with the activity of 200 u/mL and B. clausii S-4 with the activity of 20 u/mL showed the maximum decrease of disease index of scab and efficiency of induced resistance to scab of leaves and stems in cucumber seedlings were 73.7% and 80.0%, 80.6% and 86.6%, respectively, pH variation of alkaline pectinase from B. gibsonii S-2 and B. clausii S-4 showed different effects on efficiency of induced resistance to cucumber scab. At pH 8.0, both the alkaline pectinases from B. gibsonii S-2（200 u/mL ） and B. clausii S-4 （20 u/mL） showed the highest efficiency of induced resistance to cu- cumber scab in the leaves and stems, which were 64.8% and 78.5%, 75.0% and 87.8%, respectively.