文献类型：期刊文献

英文题名：Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production by Rhodospirillum rubrum Using a Two-Step Culture Strategy

作者：Liu J.; Zhao Y.; Diao M.; Wang W.; Hua W.; Wu S.; Chen P.; Ruan R.; Cheng Y.

第一作者：Liu J.

机构：[1]Beijing Key Laboratory of Biomass Waste Resource Utilization, Biochemical Engineering College, Beijing Union University, Beijing, 100023, China;[2]Center for Biorefining, Department of Bioproducts and Biosystems Engineering, University of Minnesota, Saint Paul, 55108, MN, United States

第一机构：北京联合大学生物化学工程学院生物医药系

通讯机构：[1]Beijing Key Laboratory of Biomass Waste Resource Utilization, Biochemical Engineering College, Beijing Union University, Beijing, 100023, China|[11417144]北京联合大学生物化学工程学院生物医药系;[11417]北京联合大学;[1141726]生物化学工程学院;

年份：2019

卷号：2019

外文期刊名：Journal of Chemistry

收录：Scopus(收录号：2-s2.0-85071191039)

语种：英文

摘要：Polyhydroxyalkanoates (PHAs) are microbially synthesized biopolyesters which have attracted great attentions as a new biological material, potential alternative to traditional fossil fuel-based plastic due to their biodegradability and biocompatibility. Poly-3-hydroxybutyrate (PHB) and poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are the most common members of PHAs. In this study, the nonsulfur and facultatively phototrophic bacterium Rhodospirillum rubrum was cultivated to accumulate PHA by a two-step culture strategy. Gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance spectroscopy (NMR) analyses showed that PHAs synthesized from fructose was PHBV, in which the 3HV content was 46.5 mol%, which means the better mechanical property. The molecular weight, distribution, and thermal features were characterized by gel permeation chromatography (GPC), differential scanning calorimeter (DSC), and thermo gravimetric analysis (TGA), respectively. The low PDI of 1.08 revealed the narrow and evenly molar mass distribution which shows the stable features. The high melting temperature and their other physical properties implied their potential applications. The traditional process of producing PHBV involves related carbon sources such as valeric acid. However, our study clearly described a new medium formula with fructose and a complete fermentation method to produce PHBV with a high 3HV faction and low molecular distribution. ? 2019 Jie Liu et al.