文献类型：期刊文献

中文题名：Identification of a novel hydrolase encoded by hy-1 from Bacillus amyloliquefaciens for bioremediation of carbendazim contaminated soil and food

作者：Ying Li[1];Miaomiao Chi[1];Xizhen Ge[1]

第一作者：李映

机构：[1]Beijing Key Laboratory of Biomass Waste Resource Utilization,Biochemical Engineering College,Beijing Union University,Beijing 100023,China

第一机构：北京联合大学生物化学工程学院生物医药系

年份：2019

卷号：12

期号：2

起止页码：218-224

中文期刊名：国际农业与生物工程学报（英文）

外文期刊名：International Journal of Agricultural and Biological Engineering

收录：Scopus;PubMed

基金：This work was financially supported by Joint Funding of Beijing Municipal Natural Science Foundation-Beijing Municipal Education Commission(KZ201911417049);National Key R&D Program of China(2017YFD0201105);Premium Funding Project for Academic Human Resources Development in Beijing Union University(BPHR2017DZ07).

语种：英文

中文关键词：carbendazim;bioremediation;antifungal and antibacterial pesticide;hydrolase;soil;food;pesticide contamination

摘要：Carbendazim(MBC)is an effective antifungal and antibacterial pesticide in agricultural applications.However,the MBC-contaminated soil and food are difficult to be restored.In this work,a novel MBC-hydrolase HY-1 encoded by gene hy-1 from an isolated MBC-degrading bacteria Bacillus amyloliquefaciens has been screened and identified.The 858 bp hydrolase gene was expressed in E.coli BL21 and the 32 kDa hydrolase HY-1 was purified.The purified HY-1 was able to catalyze MBC into 2-aminobenzimidazole(2-AB)without the need for any cofactors.Then bioremediation experiment was conducted and both the strain Car4 and cell crude extract of E.coli(pET-hy1)accelerated MBC degradation in soil.Moreover,purified HY-1 was available in removing MBC residue on the surface of cucumber.This work explored the possibility of microbial and enzymatic bioremediation on MBC-contaminated soil and food,provide a new way for bioremediation of pesticide contaminations.