文献类型：期刊文献

英文题名：Optimization of Promethazine and Demethylpromethazine Separation Conditions in Capillary Electrophoresis by Response Surface Methodology

作者：Gu, Chunxiu[1,2];BingHan[1];Dong, Yan[1];Liu, Bathing[1,2];Zhou, Kaowen[1,2]

第一作者：Gu, Chunxiu;谷春秀

通讯作者：Dong, Y[1];Liu, B[1];Zhou, KW[1];Liu, B[2];Zhou, KW[2]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2021

卷号：16

期号：3

起止页码：1-11

外文期刊名：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

收录：;Scopus(收录号：2-s2.0-85102103971);WOS:【SCI-EXPANDED(收录号:WOS:000629654700033)】；

基金：This work was supported by Beijing Natural Science Foundation of China (Grant No.2152013).

语种：英文

外文关键词：Capillary electrophoresis; Promethazine; Demethylpromethazine; Optimization; Response surface methodology

摘要：Demethylpromethazine (DMPMZ) is one of the main metabolites of promethazine (PMZ). DMPMZ has only one methyl less than PMZ. In the past, in order to separate them by capillary electrophoresis (CE), it is often necessary to add additives to separation buffer solution. Under the optimized experimental conditions by response surface methodology, however, PMZ and DMPMZ can be separated well without using additives. The optimum CE conditions were 21.846 mmol/L phosphate buffer solution (pH 5.653) and separation voltage of 14.408 kV. The resolution of PMZ and DMPMZ is more than 1.5 without any additives under the optimized experimental conditions. The optimization method is universal for many multiparameter processes.