文献类型：期刊文献

英文题名：Application of analytical and preparative high-speed counter-current chromatography for separation of alkaloids from Coptis chinensis Franch

作者：Yang F.[1];Zhang T.[1];Zhang R.[2];Ito Y.[3]

通讯作者：Ito, Y[1]

机构：[1]NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA;[2]Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China;[3]Beijing Union Univ, Chem Engn Coll, Beijing 100029, Peoples R China

第一机构：NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

通讯机构：[1]corresponding author), NHLBI, Biophys Chem Lab, NIH, Bldg 10, Bethesda, MD 20892 USA.

年份：1998

卷号：829

期号：1-2

起止页码：137-141

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000078080200010)】；

基金：Financial support from Beijing Academy of Science and Technology and Beijing Commission of Science and Technology is gratefully acknowledged. We also thank senior engineer Xining Li for his excellent technical assistance.

语种：英文

外文关键词：Coptis chinensis Franch; high-speed counter-current chromatography; alkaloids

摘要：Analytical high-speed counter-current chromatography (HSCCC) was used for the systematic selection and optimization of the two-phase solvent system to separate alkaloids from Coptis chinensis Franch. The optimum solvent system thus obtained led to the successful separation of alkaloids from C. chinensis Franch by preparative HSCCC. One batch separation yielded four pure alkaloids, including palmatine, berberine, epiberberine and coptisine from the crude alkaloid extract. (C) 1998 Elsevier Science B.V. All rights reserved.