文献类型：期刊文献

英文题名：Expression of the TetA gene encoding TetA efflux protein in E. coli contributes to its increased bacterial resistance toward berberine

作者：Li, Ying[1];Cao, Zhe-Tong[1];Wang, Xin-Yue[1];Ge, Xi-Zhen[1]

第一作者：李映

通讯作者：Ge, XZ[1]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2018

卷号：20

期号：4

起止页码：374-384

外文期刊名：JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000435527900010)】；

基金：This work was financially supported by the Innovation Capability Enhancement Program for universities governed by Beijing Municipal Commission of Education [number PXM 2016_014209_000024_00202762_FCG]; Beijing Natural Science Foundation [number 5164030]; Bioactive Substances and Function Food Open Issue of Beijing Municipal Key Laboratory, Beijing Union University [Zk 70201406]; Project for building virtual teaching and researching team to enhance teaching quality [number PXM 2016_014209_000023] and Project for building emerging major by joint training to enhance the quality of graduate [number PXM 2016_014209_000049].

语种：英文

外文关键词：TetA efflux protein; berberine; bacterial resistance; tetracycline; EC50

摘要：Berberine (BBR) is a traditional Chinese medicine in various applications due to its antibacterial effect. Here we investigated the increased bacterial resistance of E. coli toward BBR. The median effective concentration (EC50) of BBR against E. coli was increased when TetA efflux protein (TEP) was introduced. Sixty-five percent of the intracellular BBR was expelled and molecular docking demonstrated the intensive interaction of TEP to BBR. Finally, the combined antibacterial experiment identified that BBR acted as an inhibitor of TEP in detoxification of tetracycline. TEP is the first discovered protein that was related to the bacterial susceptibility to BBR. [GRAPHICS] .