文献类型：期刊文献

英文题名：Differential effects of electroacupuncture and manual acupuncture on spontaneously hypertensive rats: insights from intestinal microbiota and metabolomics

作者：Liu, Ji-Peng[1];Tu, Long-Teng[1];Yang, Ke-Zhen[2];Li, Yin-Yin[3];Gong, Yu[1];Han, Bing-Xuan[1];Liu, Chuan[4];Xia, Tian-Qi[4];Liu, Yu[5];Hao, Xiao-Min[1];Yue, Bing-Nan[1];Zhang, Jing[1];Wang, Bing-Hui[1];Luo, Gui-Rong[1];Liu, Qing-Guo[1];Xu, Meng[6]

第一作者：Liu, Ji-Peng

通讯作者：Liu, QG[1];Xu, M[2]

机构：[1]Beijing Univ Chinese Med, Sch Acupuncture Moxibust & Tuina, Beijing, Peoples R China;[2]Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Rehabil Med, Sch Med, Hangzhou, Zhejiang, Peoples R China;[3]Beijing Univ Chinese Med, Dept Shenzhen Hosp, Shenzhen, Peoples R China;[4]Wangjing Community Hlth Ctr, Dept Tradit Chinese Med, Beijing, Peoples R China;[5]Beijing Union Univ, Coll Special Educ, Beijing, Peoples R China;[6]Beijing Univ Chinese Med, Dept Tuina, Affiliated Hosp 3, Beijing, Peoples R China

第一机构：Beijing Univ Chinese Med, Sch Acupuncture Moxibust & Tuina, Beijing, Peoples R China

通讯机构：[1]corresponding author), Beijing Univ Chinese Med, Sch Acupuncture Moxibust & Tuina, Beijing, Peoples R China;[2]corresponding author), Beijing Univ Chinese Med, Dept Tuina, Affiliated Hosp 3, Beijing, Peoples R China.

年份：2025

卷号：12

外文期刊名：FRONTIERS IN MOLECULAR BIOSCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001533341100001)】；

基金：The author(s) declare that financial support was received for the research and/or publication of this article. This work was funded by grants from the National Natural Science Foundation of China (No. 82074553), Beijing University of Traditional Chinese Medicine Special Funds for Scientific Research Operating Expenses (No. 2024-JYB-JBZD-025), Zhejiang Provincial Natural Science Foundation of China (No. LQN25H270003) and Zhejiang Province Traditional Chinese Medicine Science and Technology Project (No. 2025ZR150).

语种：英文

外文关键词：hypertension; electroacupuncture; manual acupuncture; spontaneously hypertensive rats; gut flora; metabolites

摘要：Background Hypertension is a significant risk factor for cardiovascular disease, and acupuncture has demonstrated therapeutic effects in managing hypertension. However, the precise antihypertensive mechanisms of acupuncture require further elucidation.Methods In this study, 30 male spontaneously hypertensive rats (SHRs) and 10 male Wistar Kyoto (WKY) rats were utilized as experimental models. The SHRs were randomly assigned to three groups: the model group (Group M), the electroacupuncture group (Group EA), and the manual acupuncture group (Group MA), while the WKY rats served as the blank control group. Treatment was given every other day for 8 weeks, and systolic and diastolic blood pressures were measured every 2 weeks during the intervention period. Upon completion of the intervention, analyses of intestinal flora, as well as serum and fecal metabolomics, were conducted.Results The findings indicated that both EA and MA effectively reduced systolic and diastolic blood pressure in SHRs, with EA demonstrating a more rapid onset of blood pressure reduction. EA and MA influence the composition of intestinal microbiota in SHRs, aligning the microbial structure more closely with that of the WKY group. This modulation results in an increased abundance of beneficial bacteria, such as Blautia, and a decreased abundance of harmful bacteria, such as Helicobacter. Regarding serum metabolomics, EA and MA affect metabolic pathways involving glycerophospholipids, linoleic acid, and arachidonic acid. In terms of fecal metabolomics, both acupuncture techniques are implicated in primary bile acid biosynthesis, dopaminergic synapse function, and sphingolipid signaling pathways. Notably, EA exerts a more significant influence on the steroid hormone biosynthesis pathway, whereas MA impacts the tryptophan metabolic pathway.Conclusion Both EA and MA demonstrate antihypertensive effects by modulating intestinal microbiota composition and metabolite profiles in SHRs, although the specific microbiota and metabolites affected differ between the two techniques, and EA treatment reversed more fecal and serum metabolites than did MA. This study serves as a reference for investigating the mechanisms underlying acupuncture in the treatment of hypertension and facilitates its clinical application.