文献类型：期刊文献

英文题名：The metabolic pathways of carbon assimilation and polyhydroxyalkanoate production by Rhodospirillum rubrum in response to different atmospheric fermentation

作者：Tang, Manyu[1];Zhen, Xin[1];Zhao, Guoqiang[1];Wu, Shuang[1,2];Hua, Wei[1,2];Qiang, Jingwen[1];Cheng, Yanling[1,2];Wang, Wanqing[1,2]

第一作者：Tang, Manyu

通讯作者：Cheng, YL[1];Wang, WQ[1];Cheng, YL[2];Wang, WQ[2]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing, Peoples R China;[2]Beijing Key Lab Biomass Waste Resource Utilizat, Beijing, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing, Peoples R China;[2]corresponding author), Beijing Key Lab Biomass Waste Resource Utilizat, Beijing, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2024

卷号：19

期号：7

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001277539200022)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (31600208); The Project of Cultivation for young top-motch Talent of Beijing Municipal Institutions (BPHR202203210).

语种：英文

摘要：The purple nonsulfur bacteria, Rhodospirillum rubrum, is recognized as a potential strain for PHAs bioindustrial processes since they can assimilate a broad range of carbon sources, such as syngas, to allow reduction of the production costs. In this study, we comparatively analyzed the biomass and PHA formation behaviors of R. rubrum under 100% CO and 50% CO gas atmosphere and found that pure CO promoted the PHA synthesis (PHA content up to 23.3% of the CDW). Hydrogen addition facilitated the uptake and utilization rates of CO and elevated 3-HV monomers content (molar proportion of 3-HV up to 9.2% in the presence of 50% H-2). To elucidate the genetic events culminating in the CO assimilation process, we performed whole transcriptome analysis of R. rubrum grown under 100% CO or 50% CO using RNA sequencing. Transcriptomic analysis indicated different CO2 assimilation strategy was triggered by the presence of H-2, where the CBB played a minor role. An increase in BCAA biosynthesis related gene abundance was observed under 50% CO condition. Furthermore, we detected the alpha-ketoglutarate (alpha KG) synthase, converting fumarate to alpha KG linked to the alpha KG-derived amino acids synthesis, and series of threonine-dependent isoleucine synthesis enzymes were significantly induced. Collectively, our results suggested that those amino acid synthesis pathways represented a key way for carbon assimilation and redox potential maintenance by R. rubrum growth under syngas condition, which could partly replace the PHA production and affect its monomer composition in copolymers. Finally, a fed-batch fermentation of the R. rubrum in a 3-l bioreactor was carried out and proved H-2 addition indeed increased the PHA accumulation rate, yielding 20% ww(-1) PHA production within six days.