文献类型：期刊文献

中文题名：姜黄素对运动性肾缺血再灌注损伤大鼠肾组织中炎症因子蛋白及基因表达的影响

英文题名：Effects of Curcumin on the Protein and Gene Expression of Inflammatory Cytokines in Renal Tissue of Rat with Exercise-related Renal Ischemia-reperfusion Injury

作者：周海涛[1,2];曹建民[3];米生权[1,2];张静[1,2];李浩[1]

第一作者：周海涛

机构：[1]北京联合大学生物化学工程学院;[2]北京联合大学生物活性物质与功能食品北京市重点实验室;[3]北京体育大学运动人体科学学院

第一机构：北京联合大学生物化学工程学院

年份：2018

卷号：30

期号：10

起止页码：1687-1694

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：北京市朝阳区协同创新项目(CYXC1508);北京市高等学校高水平人才交叉培养"实培计划"

语种：中文

中文关键词：姜黄素;过度训练;运动性肾缺血再灌注损伤;炎症因子

外文关键词：Curcumin;kidney,Overtraining;exercise-induced ischemia-reperfusion;inflammatory factors

摘要：本文以姜黄素对过度训练所致运动性肾缺血再灌注损伤大鼠肾脏病理改变,炎症因子蛋白及基因表达的影响为目的进行了研究。72只7周龄SPF级雄性Wistar大鼠随机分为安静对照组(C)、一般训练组(M)、过度训练组(OM)和姜黄素+过度训练组(COM)。实验中OM、COM组进行8周递增负荷游泳训练,COM组大鼠以200 mg/kg/d、5 m L/kg姜黄素灌胃56 d,其他组给予等体积0. 5%的羧甲基纤维素纳灌胃。末次训练24 h后处死大鼠,光镜和电镜观察肾组织形态和超微结构改变;测定血清肌酐、尿素氮;肾组织IL-1β、TNF-α、IL-6基因和蛋白表达。结果显示:8周的递增负荷游泳训练后,光镜下C、M组大鼠肾组织结构正常; OM组出现组织病理学改变; COM组较OM组明显减轻。Paller评分,OM组显著高于C组,COM组显著低于OM组。电镜下C组结构正常; M组结构出现轻微改变; OM组结构改变明显; COM组较M组病变程度明显减轻。血清肌酐、尿素氮水平,OM组显著高于C组,COM组显著低于OM组。OM组肾组织IL-1β、TNF-α、IL-6蛋白表达显著高于C组,COM组显著低于OM组; OM组肾组织IL-1β、TNF-α和IL-6 mRNA表达上调显著高于C组,COM组显著低于OM组。血清睾酮/皮质酮比值,OM组显著低于C组。从而说明8周的游泳训练导致大鼠出现过度训练及运动性肾缺血再灌注损伤,肾脏发生病理改变。姜黄素可以延缓过度训练的发生与发展,通过抑制炎性因子的蛋白和基因表达,预防和延缓过度训练导致的运动性肾缺血再灌注损伤的发生与发展,有效地改善了大鼠肾脏功能,缓解组织形态学及超微结构改变。
In this paper,the effects of curcumin on the histomorphology,ultrastructure,protein and gene expression of inflammatory factors in the kidney of rats with exercise-related renal ischemia-reperfusion injury was studied.72 SPF male Wistar rats at the age of 7-week-old were divided into 4 groups randomly:control group(C),general training group(M),overtraining group(OM),curcumin+overtraining group(COM).The rats in OM,COM groups were assigned under progressively increasing load training for 8 weeks,while the rats of COM group were given 200 mg/kg/d of curcumin in the volume as 5 mL/kg,other groups were given the same volume of 0.5%sodium carboxymethyl cellulose.Kill the rats 24 h after the last training,the renal tissue morphological was conducted by optical and electronic microscopy;serum creatinine and blood urea nitrogen was measured.Protein and gene of IL-1β,TNF-αand IL-6 in renal tissue were detected.The results showed:after progressively increasing load training for 8 weeks,renal tissue structures of C group and M group appear normal in pathology slice;histopathological abnormalities appear in OM group;the pathological changes of kidney tissue in COM group were significantly less than those in OM group.Paller score of OM group was significantly higher than that in C group,COM group was significantly lower than that of OM group.Under electron microscope,the structure of C group were normal;that of M group change slightly;OM group change obviously,and the pathological changes of COM group was significantly reduced compared to M group.Blood urea nitrogen and serum creatinine of OM group were significantly higher than those of C group,and those two in COM group were significantly lower than in OM group; IL-1β,TNF-αand IL-6 protein of ranal tissue in OM group was significantly higher than that in C group,and those proteins in COM group was significantly lower than those of OM group;for the expression of OM group,renal IL-1βmRNA,TNF-αmRNA and IL-6 mRNA were significantly higher than those in C group,but in COM group were lower than in OM group.Serum testosterone/corticosterone ratio in group OM was significantly lower than that in group C.The 8 weeks progressively increasing-load swimming training resulted in overtraining and exercise-induced renal ischemia-reperfusion injury in rats,accompanied by ultrastructure and pathological changes in the kidney.Curcumin can significantly reduce renal dysfunction and kidney tissue ultrastructure of the extent of the damage and the histopathological changes,which may be achieved by inhibiting the expression of inflammatory cytokines IL-1β,TNF-αand IL-6,resistance to various stress stimuli,as a result,curcumin can protect the rats from exercise-related renal ischemia-reperfusion injury,effectively improved the renal function,and alleviated the morphological and ultrastructural changes.