文献类型：期刊文献

英文题名：In vitro antioxidant activities of Rhodobacter sphaeroides and protective effect on Caco-2 cell line model

作者：An, Jun[1,2]; Yang, Cui[1]; Li, Zuming[1]; Finn, Patricia W.[3]; Perkins, David L.[3]; Sun, Jun[3]; Bai, Zhihui[4]; Gao, Liping[1]; Zhang, Michael[5]; Ren, Difeng[2]

第一作者：An, Jun

通讯作者：Li, Zuming|[1141715fcadfa50747025]李祖明;

机构：[1] Department of Food Science and Beijing Key Laboratory of Bioactive Substances and Functional Foods, College of Biochemical Engineering, Beijing Union University, Beijing, China; [2] Beijing Key Laboratory of Forest Food Processing and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China; [3] Department of Medicine, University of Illinois at Chicago, Chicago, IL, United States; [4] Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China; [5] Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]Department of Food Science and Beijing Key Laboratory of Bioactive Substances and Functional Foods, College of Biochemical Engineering, Beijing Union University, Beijing, China|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2019

卷号：103

期号：2

起止页码：917-927

外文期刊名：Applied Microbiology and Biotechnology

收录：EI(收录号：20184606072895);Scopus(收录号：2-s2.0-85056353016)

语种：英文

外文关键词：Antioxidants - Cell culture - Enzymes - Oxidative stress - Probiotics

摘要：The present study aimed to evaluate the in vitro antioxidant activities and the protective effect of Rhodobacter sphaeroides on H2O2-induced oxidative stress in Caco-2 cells. The results showed that the antioxidant action of R. sphaeroides varied with different cell concentrations and treatments. Also, the intact cells and intracellular cell-free extracts showed better antioxidant activities. Caco-2 cell–based oxidative stress model was developed by optimizing H2O2 concentration and culture time with the half lethal dose and methyl thiazolyl tetrazolium. By increasing the activity of endogenous antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, upregulating the antioxidant ability of the anti-superoxide anion and anti-hydroxyl radical, R. sphaeroides, especially the mutant strain R. sphaeroides (CGMCC No. 8513), exhibited significant protective activity against H2O2-induced oxidative stress in Caco-2 cells. Taken together, R. sphaeroides (CGMCC No. 8513) exhibits strong antioxidant activities and is a candidate to be investigated as a potential probiotic in the future. ? 2018, Springer-Verlag GmbH Germany, part of Springer Nature.