文献类型：期刊文献

英文题名：Screening of Potential Probiotic Lactobacillaceae and Their Improvement of Type 2 Diabetes Mellitus by Promoting PI3K/AKT Signaling Pathway in db/db Mice

作者：Li, Yueyang[1];Tong, Tong[1];Li, Peifan[1];Peng, Yian[1];Zhang, Michael[2,3];Liu, Jia[4];She, Yongxin[5];Li, Zuming[1];Li, Yongli[6]

通讯作者：Li, ZM[1];Li, YL[2]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China;[2]Univ Manitoba, Dept Phys & Astron, Winnipeg, MB, Canada;[3]Anhui Univ Sci & Technol, Sch Publ Hlth, Hefei, Peoples R China;[4]Internal Trade Food Sci & Technol Beijing Co Ltd, Beijing, Peoples R China;[5]Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing, Peoples R China;[6]Zhengzhou Univ, Henan Prov Peoples Hosp, Peoples Hosp, Zhengzhou, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China;[2]corresponding author), Zhengzhou Univ, Henan Prov Peoples Hosp, Peoples Hosp, Zhengzhou, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2023

卷号：72

期号：3

起止页码：285-297

外文期刊名：POLISH JOURNAL OF MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001071906800006)】；

基金：This work was supported by the Natural Science Foundation of Beijing Municipality under Grant No. 6173033; Academic Research Projects of Beijing Union University under Grant No. ZK20202307; Sino Canada Health Engineering Research Institute cooperation projects under Grant No. 50304JA1901; Internal Trade Food Science and Technology (Beijing) Co., Ltd. cooperation projects under Grant No. 202116.

语种：英文

外文关键词：Lactobacillaceae; type 2 diabetes mellitus; PI3K/AKT pathway; probiotics properties; short chain fatty acids

摘要：The study aimed to isolate Lactobacillaceae strains with in vitro hypoglycemic activity and probiotic properties and to determine their antidiabetic abilities in vivo. Lactiplantibacillus plantarum 22, L. plantarum 25, Limosilactobacillus fermentum 11, and L. fermentum 305 with high in vitro hypoglycemic activity were screened from 23 strains of Lactobacillaceae isolated from human feces and identified by 16S rDNA sequencing. The fasting blood glucose (FBG) of the mice was recorded weekly. After 12 weeks, liver, kidney, and pancreas tissues were stained with hematoxylin and eosin (H&E) to observe histomorphology; the inflammatory factors were assayed by Quantitative Real-time PCR; PI3K and AKT were measured by Western blot; the short-chain fatty acids (SCFAs) were determined by LC-MS/MS. Inhibitory activities of L. plantarum 22, L. plantarum 25, L. fermentum 11, and L. fermentum 305 against alpha-amylase were 62.29 +/- 0.44%, 51.81 +/- 3.65%, 58.40 +/- 1.68%, and 57.48 +/- 5.04%, respectively. Their inhibitory activities to alpha-glucosidase were 14.89 +/- 0.38%, 15.32 +/- 0.89%, 52.63 +/- 3.07%, and 51.79 +/- 1.13%, respectively. Their survival rate after simulated gastrointestinal test were 12.42 +/- 2.84%, 9.10 +/- 1.12%, 5.86 +/- 0.52%, and 8.82 +/- 2.50% and their adhesion rates to Caco-2 cell were 6.09 +/- 0.39%, 6.37 +/- 0.28%, 6.94 +/- 0.27%, and 6.91 +/- 0.11%, respectively. The orthogonal tests of bacterial powders of the four strains showed that the maximum inhibitory activities to alpha-amylase and alpha-glucosidase were 93.18 +/- 1.19% and 75.33 +/- 2.89%, respectively. The results showed that the mixture of Lactobacillaceae could lower FBG, reduce inflammation, and liver, kidney, and pancreas damage, promote PI3K/AKT signaling pathway, and increase the content of SCFAs. The combination of L. plantarum 22, L. plantarum 25, L. fermentum 11, and L. fermentum 305 can potentially improve type 2 diabetes mellitus (T2DM).