文献类型：期刊文献

英文题名：A Chemical Investigation of the Leaves of Morus alba L.

作者：Chen, Xiao-yan[1];Zhang, Ting[2,3];Wang, Xin[4];Hamann, Mark T.[1];Kang, Jie[5,6];Yu, De-quan[5,6];Chen, Ruo-yun[5,6]

第一作者：Chen, Xiao-yan

通讯作者：Chen, RY[1];Chen, RY[2]

机构：[1]Med Univ South Carolina, Dept Drug Discovery & Biomed Sci, Coll Pharm, Charleston, SC 29425 USA;[2]Chinese Acad Med Sci, Inst Med Informat & Lib, Beijing 100020, Peoples R China;[3]Peking Union Med Coll, Beijing 100020, Peoples R China;[4]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China;[5]Chinese Acad Med Sci, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Beijing 100050, Peoples R China;[6]Peking Union Med Coll, Beijing 100050, Peoples R China

第一机构：Med Univ South Carolina, Dept Drug Discovery & Biomed Sci, Coll Pharm, Charleston, SC 29425 USA

通讯机构：[1]corresponding author), Chinese Acad Med Sci, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Beijing 100050, Peoples R China;[2]corresponding author), Peking Union Med Coll, Beijing 100050, Peoples R China.

年份：2018

卷号：23

期号：5

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000435204000033)】；

基金：The author is grateful to Li Li who helped measure the CD spectra, Zhu-Fang Shen for the aldose reductase assay and Nai-Hong Chen for the neuroprotection bioassay. This work was supported by CAMS Innovation Fund for Medical Sciences (CIFMS) (2016-I2M-1-010).

语种：英文

外文关键词：Morus alba L; aldose reductase inhibitor; neuroprotective agent; natural products

摘要：The leaves of Morus alba L. are an important herbal medicine in Asia. The systematic isolation of the metabolites of the leaves of Morus alba L. was achieved using a combination of liquid chromatography techniques. The structures were elucidated by spectroscopic data analysis and the absolute configuration was determined based on electronic circular dichroism (ECD) spectroscopic data and hydrolysis experiments. Their biological activity was evaluated using different biological assays, such as the assessment of their capacity to inhibit the aldose reductase enzyme; the determination of their cytotoxic activity and the evaluation of their neuroprotective effects against the deprivation of serum or against the presence of nicouline. Chemical investigation of the leaves of Morus alba L. resulted in four new structures 1-4 and a known molecule 5. Compounds 2 and 5 inhibited aldose reductase with IC50 values of 4.33 M and 6.0 M compared with the potent AR inhibitor epalrestat (IC50 1.88 x 10(-3) M). Pretreatment with compound 3 decreased PC12 cell apoptosis subsequent serum deprivation condition and pretreatment with compound 5 decreased nicouline-induced PC12 cell apoptosis as compared with control cells (p < 0.001).