文献类型：期刊文献

英文题名：Evaluation of berberine as a natural compound to inhibit peach brown rot pathogen Monilinia fructicola

作者：Fu, Weihui[1];Tian, Geran[3];Pei, Qinghui[2];Ge, Xizhen[2];Tian, Pingfang[1]

第一作者：Fu, Weihui

通讯作者：Tian, PF[1];Ge, XZ[2]

机构：[1]Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China;[2]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[3]Cornell Univ, Coll Agr & Life Sci, Ithaca, NY 14853 USA

第一机构：Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China

通讯机构：[1]corresponding author), Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China;[2]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2017

卷号：91

起止页码：20-26

外文期刊名：CROP PROTECTION

收录：;Scopus(收录号：2-s2.0-84988516585);WOS:【SCI-EXPANDED(收录号:WOS:000388783300004)】；

基金：This work was supported by grants from the Innovation Capability Enhancement Program for universities Governed by Beijing Municipal Commission of Education (No. PXM2015_014209_000010), Project from Beijing Key Laboratory of Bioactive Substances and Functional Foods in Beijing Union University (ZK70201406), National High Technology Research and Development Program (863 Program) (No. 2015AA021003), National Basic Research Program of China (973 Program) (No. 2012CB725200), National Natural Science Foundation of China (No. 21276014, 21476011), and Fundamental Research Funds for the Central Universities (YS1407).

语种：英文

外文关键词：Berberine; Monilinia fructicola; 50% effective concentration; Resistance; Field trial

摘要：Berberine (BBR) is a traditional antidiarrheal compound with excellent safety profiles. Our previous study reported that BBR strongly inhibited brown rot pathogen Monilinia fructicola. In this study we assess its potential commercial performance. We found that the spore germination and mycelial growth of M. fructicola could be totally inhibited in vitro by BBR at 4.10 mu g ml(-1) and 45.50 mu g ml(-1), respectively, suggesting the strategy of spraying BBR prior to hyphal growth. Next, we compared the 50% effective concentration (EC50) and minimal inhibitory concentration (MIC) of BBR with those of synthetic chemical fungicides, and results showed that BBR is economically viable to manage brown rot disease. Strikingly, although M. fructicola was successively treated by BBR for sixteen generations, the EC50 and MIC values remained nearly unchanged, and no mutation occurred in the genes that confer resistances to chemical fungicides, indicating that M. fructicola is less likely to develop resistance to BBR. Furthermore, two years of field trials showed that BBR effectively controlled peach brown rot and no visual damage was observed on leaves and fruits. Given its well-known safety, high efficacy and prolonged activity, we believe that BBR is a promising natural fungicide to control brown rot disease. (C) 2016 Elsevier Ltd. All rights reserved.